In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.

某些结构，如线粒体基粒、核糖体和蛋白质及其组装成的纤维甚至病毒等，可以通过负染色（negative staining）电镜技术观察其精细结构。 其分辨率可达1.5nm左右。

2022年8月10日 · The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed. It is also used to prepare biological samples for electron microscopy.

负染色法（Negative stain）是一种染色方法，常用于不透光液体标本的镜检。 由于其染色处理过程并非针对菌体本身，故又称“ 衬托染色法 ”、“ 间接染色法 ” [ 1 ] 。

负染色法（Negative stain）是一种染色方法，常用于不透光液体标本的镜检。 由于其染色处理过程并非针对菌体本身，故又称“衬托染色法”、“间接染色法”。

Negative staining refers to a rapid, uncomplicated and qualitative staining technique that examines bacterial morphological characteristics. It is named negative staining because it uses negative or acidic stains that do not bind to the test specimen.

In negative stain, the noise levels are so low that this detection often fails and zeroes out high contrast pixels which should be retained. Normally people who know negative stain well, and have nicely stained images will collect their images at or near Scherzer defocus (ie - …

Negative staining, the embedding of a specimen in a layer of dried heavy metal solution, was introduced early on as a quick and easy specimen preparation technique that significantly increases...

2021年6月5日 · Principle of Negative Staining. Negative staining requires the use of an acidic stain such as India ink or nigrosin. The acidic stain, with its negatively charged chromogen, will not penetrate the cells because of the negative charge on the surface of bacteria. Therefore, the unstained cells are easily discernible against the colored background.

2025年1月22日 · One common negative staining technique for identifying encapsulated yeast and bacteria is to add a few drops of India ink or nigrosin to a specimen. Other capsular stains can also be used to negatively stain encapsulated cells (Figure \(\PageIndex{7}\)). Alternatively, positive and negative staining techniques can be combined to visualize ...