In this study we used TTC, Fluoro-Jade B, and NeuN staining to examine the chronology of infarct development following MCAO. Our objectives were to determine if Fluoro-Jade B was superior to TTC staining, to delineate the time course of infarct progression, and to establish the anatomic boundaries of core and penumbra in mice at several time ...

2019年1月24日 · Low and high scale mapping of the glial activation on brain sections of mice subjected to 30 minutes middle cerebral artery occlusion (MCAO) was correlated with that of the neuronal cell death,...

To compare tissue viability at both acute and sub-acute time points, we used 2,3,5-triphenyltetrazolium chloride (TTC), Fluoro-Jade B, and NeuN staining to examine the evolving phases of infarction induced by a 90-min MCAO in mice. Stroke outcomes were examined at 1.5h, 6h, 12h, 24h, 3d, and 7d after MCAO.

... immunoreactivity was assessed by NeuN immunofluorescence staining. As presented in Fig. 4, the MCAO group exhibited significantly decreased NeuN expression compared with the sham...

神经元核抗原(NeuN)是成熟神经元细胞的标记，MCAO大鼠NeuN减少，是神经元特异质基因的改变 。从免疫荧光染色结果中可见pOBz给药2d对MCAO大鼠NeuN蛋白表达在形态和数量上都有明显的改善。

免疫荧光结果显示，经大黄提取物治疗以后可以增加缺血侧脑组织NeuN的表达，表明大黄提取物对于MCAO模型大鼠的神经元损伤具有抑制作用。

Glial (GFAP+) and neuronal nuclei (NeuN+) activity after MCAO. Double immunostaining of GFAP/NeuN at edges of lesion core of control (a), day 5 (b), day 7 (c), day 11 (d), and day 18 (e).

It has been reported that the staining signal of NeuN-positive cells is weak but still present 24 h after a focal stroke, resulting in pyknosis 5, 35 . We also found that only about 80% of NeuN...

经Sal连续治疗6 d以后，能够改善pMCAO模型大鼠脑组织缺血侧NeuN及NGF蛋白的表达，表明Sal对pMCAO模型大鼠具有神经保护作用，此作用与促进NGF蛋白的表达，进而提升神经细胞存活量有关。

2022年9月23日 · For evaluating MCAO-induced neural damage, we performed immunostaining using various neuronal markers (NeuN, parvalbumin [PV], and microtubule-associated protein 2 [MAP2]) and a glial marker ...