The effect of LPS stimulation on NFAT2 and HMGB1 interaction in the cytoplasm was observed by immunoprecipitation assay. HMGB1 production and release was detected in cells with specific small interfering RNA (siRNA)-mediated suppression of NFAT2 expression. Results LPS stimulated HMGB1 release from THP-1 cells. As LPS stimulation prolonged ...

2022年11月3日 · In a mouse model of LPS-induced acute lung injury, ROS produced by NOX2 promoted stromal interaction molecule1 dependent (STIM1-dependent) cytosolic Ca 2+ oscillations and subsequent NFAT activation, and this STIM1-mediated NFAT activity was necessary for LPS-induced proinflammatory gene expression in endothelial cells .

活化的T细胞核内因子（Nuclear Factors of activated T cells， NFAT）是一类 转录因子 家族，最初是在活化的T细胞中作为一种能够结合在人白介素-2（Interleukin-2，IL-2）的启动子上的快速诱导其表达的转录因子而被发现。 NFAT在多种动物组织和细胞中广泛表达，是细胞内多条 信号转导通路 的关键调节点，在免疫系统、神经系统发育、轴突生长以及神经系统疾病中也发挥着重要作用。 NFAT蛋白的基本结构.

2024年3月13日 · 活化T细胞核因子2（NFAT2）是一种Ca2+依赖的转录因子，调节直接指导CD8+ T细胞效应功能的基因表达。 磷酸化形式（不活跃）的NFAT2位于细胞质中。 在T细胞受体（TCR）刺激下，细胞内Ca2+的升高激活了磷酸酶钙调磷酸酶，使细胞质中的NFAT2去磷酸化，导致其转运到细胞核中，并在那里转录激活多种效应分子。 在结直肠癌中，CD8+ T细胞浸润与患者的生存率相关。 高水平的浸润性CD8+ CD45RO+ T细胞的存在，以及编码CD8、IFN-γ …

VIVIT attenuated LPS or LPS plus IFN-γ induced IL-12 p40 mRNA and protein expression in bone marrow derived macrophages (BMDMs) and reduced DNA binding of NFAT to a composite NFAT–interferon stimulated response element (ISRE) on the Il12b promoter. In addition, VIVIT inhibited the production of NO and the secretion of IL-12 p70, IL-23, and ...

2014年1月30日 · CD14 was also shown to induce Ca 2+ influx and NFAT activation causing apoptosis of differentiated DCs, but not macrophages, stimulated by LPS. NFAT2/c1 activated by the receptor activator of NF-κB (RANKL) was also shown to play a key regulatory role in the terminal differentiation of osteoclasts.

2016年4月21日 · In this review, we address the possible reasons for these distinct roles, particularly regarding N- and C-terminal transactivation regions (TADs) and the partner proteins that interact with these...

2012年8月16日 · Numerous studies have demonstrated that LPS binding to CD14 is enhanced by LPS binding protein, which is followed by CD14-mediated transfer of LPS to the TLR4/MD2 complex and subsequent activation of the intracellular cascade through adaptors MyD88-TIRAP and TRAM-TRIF.

The presence of the β-specific sequence is associated with both quantitative and qualitative differences in transactivation ability: NFAT2.β demonstrates a greater ability to transactivate in response to both PMA/ionomycin and LPS over a range of …

The effect of LPS stimulation on NFAT2 and HMGB1 interaction in the cytoplasm was observed by immunoprecipitation assay. HMGB1 production and release was detected in cells with specific small interfering RNA (siRNA)-mediated suppression of NFAT2 expression. Results: LPS stimulated HMGB1 release from THP-1 cells. As LPS stimulation prolonged ...