NotI has a High Fidelity version NotI-HF ® (NEB #R3189). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

NotI-HF® has the same specificity of NotI (NEB #R0189), but it has been engineered for reduced star activity. Blocked by CpG methylation. Protocols, Manuals & Usage

Thermo Scientific NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37C in O buffer (Isoschizomers: CciNI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, op

本酶提供高保真版 NotI-HF ® （NEB #R3189）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。

We are currently designing a range of viral vectors containing a NotI site upstream of SbfI and PacI sites. These sites allow the direct cloning of your SnapFast genes into these viral vectors. The NotI site is designed to be used for inserting genes into viruses where a promoter is not required (e.g. RNA viruses or viruses already containing a ...

碧云天自主研发生产的NotI，是从大肠杆菌表达纯化获得的一种限制性内切酶[1]，是CciNI的同裂酶(Isoschizomers)，其基本信息如下：

Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated. Incubation Conditions: Buffer D. 37°C. Source: Nocardia otitidis-caviarum.

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Thermo Scientific NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37C in O buffer (Isoschizomers: CciNI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, op

2008年4月8日 · NotI is a dimer, as commonly observed for orthodox restriction enzymes, which interacts with palindromic recognition sites. Each subunit shows a typical restriction endonuclease-like fold, consisting of a central five-stranded β sheet flanked by two α helices. The conserved core harbors the canonical PD… (D/E)xK-like catalytic motif.