Critically, Nox4 has an unusually high K m for oxygen (∼18%), similar to the values of known oxygen-sensing enzymes, compared with a K m of 2–3% for Nox2, the phagocyte NADPH oxidase. This allows Nox4 to generate H 2 O 2 as a function of oxygen concentration throughout a physiological range of pO 2 values and to respond rapidly to changes ...

As a key oxygen sensor, Nox4-derived H2O2 plays diverse roles in cell proliferation, migration and death. Increased expression of Nox4 in cancer has been observed, which participates in metastasis, angiogenesis and apoptosis.

2019年3月5日 · We identified a source of H 2 O 2, the enzyme NADPH oxidase (NOX) 4, in isolated differentiated, in-vitro fertilisation-derived human granulosa-lutein cells (GCs), in...

2012年3月27日 · Nox4 is highly expressed in the vasculature and it generates mostly H 2 O 2. Using Nox4-knockout mice, we demonstrate that Nox4-derived H 2 O 2 maintains endothelial expression of the protective endothelial enzymes HO-1 and eNOS. We found that loss of Nox4 potentiates the proinflammatory and prohypertrophic effect of angiotensin II and ...

2023年6月23日 · It is unique among Nox enzymes in its lack of required cytosolic regulatory subunits and a propensity to produce hydrogen peroxide (H 2 O 2) rather than superoxide. Nox4 has multiple subcellular localizations depending on cell type and environmental context.

Using a ratiometric H (2)O (2) sensor targeted to different subcellular compartments, we demonstrate specific production of H (2)O (2) by the ER in response to the stressors tunicamycin and HIV-1 Tat, but not to thapsigargin or dithiothreitol.

2014年8月1日 · 我们发现大约 90% 的电子通量通过孤立的 Nox4 产生 H 2 O 2并且 10% 形成超氧化物。H的动力学机制2 O 2 的形成与一个氧分子结合的机制一致，然后在两个单电子还原步骤中被血红素依次还原，首先形成结合的超氧化物中间体，然后形成 H 2 O 2；动力

在本研究中，发现葡萄糖剥夺可诱导 HepG2 细胞中产生细胞内过氧化氢 (H2O2)。 N-乙酰半胱氨酸 (NAC) 是一种抗氧化试剂，可减少葡萄糖剥夺引起的细胞 H2O2 水平的增加和 AKT 磷酸化。 这些结果强烈表明细胞中葡萄糖剥夺诱导的 H2O2 增加介导了 AKT 磷酸化。 NOX4的RNA干扰，而不是NOX5的RNA干扰，完全抑制葡萄糖剥夺诱导的 AKT 磷酸化以及细胞内 ROS 水平的增加，而外源性 H2O2 仍然可以诱导 NOX4 敲低细胞中的 AKT 磷酸化。 在这项研究中，我们证明了 …

2023年11月20日 · Here we show that hypoxia upregulates the Nox4 protein level and Nox4-catalyzed H 2 O 2 production without increasing the Nox4 mRNA in rat H9c2 cardiomyocytes. In these cells, the Nox4 protein is stabilized under hypoxic conditions in a manner dependent on the presence of p22 phox.

2014年8月12日 · Critically, Nox4 has an unusually high Km for oxygen (∼18%), similar to the values of known oxygen-sensing enzymes, compared with a Km of 2-3% for Nox2, the phagocyte NADPH oxidase. This allows Nox4 to generate H₂O₂ as a function of oxygen concentration throughout a physiological range of pO2 values and to respond rapidly to changes in pO₂.