Sodium taurocholate cotransporting polypeptide is a functional …
2012年11月13日 · Both human NTCP (hNTCP) and tsNTCP could be efficiently cross-linked by WT b but not N9K b when expressed in 293T cells as shown by Western blotting with the anti …
Human NTCP transgenic (hNTCP-Tg) C57BL/6 mice. (A
Expression of hNTCP transgene was detected by a monoclonal antibody (1D4) that specifically recognizes the C—terminal tag (C9) of human NTCP transgene. Mouse GAPDH was used as …
Woodchuck sodium taurocholate cotransporting polypeptide supports low ...
2017年5月1日 · HepG2 cells stably-expressing wNTCP (labeled in figures as “wNTCP stable”) was established by transfection of HepG2 cells with a plasmid (pcDNA6) encoding wNTCP …
The p.Ser267Phe variant of sodium taurocholate cotransporting ...
2018年9月1日 · HepG2 cells were transfected with plasmids expressing NTCP-WT or NTCP-S267F fusing with a 9 amino acids tag at C-terminus (C9). After resistance screened and …
Hepatitis D Virus Infection of Mice Expressing Human Sodium
2015年4月22日 · To generate a mouse model for HDV infection, we created C57BL/6 mouse lines expressing hNTCP with a C—terminal tag (C9), driven by a mouse albumin …
H11-pAlb-NTCP-C9-IRES-EGFP - 南模生物
图 2 免疫组化染色结果显示，在H11 Alb-NTCP-C9 tag-IRES-EGFP/+ 小鼠的肝部可见C9 tag 的表达。 Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导 …
Molecular Determinants of Hepatitis B and D Virus Entry …
Human and mouse NTCP were amplified using cDNA from primary human or mouse hepatocytes, respectively, and cloned into pcDNA6 vector with a C9 tag at the C terminus. NTCP chimeras …
An E. coli-produced single-chain variable fragment (scFv) targeting ...
2019年2月1日 · A HepG2 cell line stably expressing C9-tagged NTCP was established by transfection with SalI-linearized pCDNA6-NTCP-C9 and subsequent selection with 5 μg/ml …
Figures and data in Sodium taurocholate cotransporting ... - eLife
2012年11月13日 · For cell surface expression, cell lysates were pulled down with streptavidin T1 Dynabeads and subsequently examined by western blot with mAb 1D4 recognizing a C9 tag at …
NTCP转运体在药物研发中的应用简介 - 药明康德DMPK
2025年1月13日 · NTCP是乙肝病毒和丁肝病毒的共同受体，介导病毒入侵肝细胞；其对胆汁酸（盐）在体内的调控发挥着重要的作用，主要转运胆盐和硫酸化化合物，同时也能够介导其它 …