2025年1月1日 · Discuss the implications of poor oxygen control on cellular O2 regulation mechanisms, bioenergetics, and redox signaling. Proposes and updated methodology on best practices for oxygen-controlled cell culture.

Here, we describe a novel in vitro system that utilizes hypoxic chambers and oxygen-permeable culture dishes to control pericellular oxygen levels and provide rapid oxygen delivery to adherent cells. This procedure is particularly relevant for protocols studying effects of rapid oxygen changes or intermittent hypoxia on cellular behavior.

Cells have a wide range of oxygen utilization, depending on cell type, function, and biological status. Measured rates of oxygen utilization by mammalian cells in culture range from <1 to >350 amol cell -1 s -1.

Specifically designed and rigorously tested for accuracy and optimal performance, our Anesthesia O 2 Cells are the only approved devices for use with GE HealthCare anesthesia machines. They allow clinicians to accurately measure and monitor the level of oxygen concentration that is inhaled and exhaled by a patient connected to an anesthesia ...

O2 sensing is a fundamental biological process necessary for adaptation of living organisms to variable habitats and physiological situations. Cellular responses to hypoxia can be acute or chronic. Acute responses rely mainly on O2-regulated ion channels, which mediate adaptive changes in cell excit …

Oxygen availability is often a limiting factor for cell survival, and it is generally supplied to a cell by passive diffusion. As oxygen molecules diffuse into the cell, they are consumed, so that there is a progressive fall in oxygen concentration from the surface of the cell to the lowest concentration which occurs at the center of the cell.

2017年12月1日 · Oxygen (O 2) is an often overlooked variable in cell and tissue culture systems. We discuss differences between in vivo and in vitro O 2 diffusion. Alternative culturing technology to enhance O 2 delivery are highlighted. Guidelines to improve cell culture quality and minimize O 2 fluctuations are proposed.

To celebrate the 2019 Nobel Prize in Physiology or Medicine, awarded to William G. Kaelin Jr., Sir Peter J. Ratcliffe, and Gregg L. Semenza for their discoveries of how cells sense and adapt to oxygen availability, we’ve asked four researchers in the oxygen-sensing field what they see on the horizon after this momentous milestone.

O 2 is a small, gaseous, non-polar analyte which has moderate solubility in aqueous solutions (pO 2 = 160 mmHg, or ~200 μM at 37°C). It is supplied to cells and tissues by passive diffusion and, in higher multicellular organisms, by convectional transport via vasculature, red blood cells and haemoglobin [3].

We offer a broad assortment of reliable, electrochemical galvanic O2 cells - ready for quick delivery and competitively priced for high-volume purchasing. O2 cells are used in life-supporting oxygen sensor monitors such as anesthesia machines, ventilators, and …