Recombinant Human N-Glycosylase/OGG1 是一种DNA修复酶，其作为一种修饰酶, 主要具有DNA糖基化酶活性和脱嘌呤/脱嘧啶（apurinic/apyrimidinic，AP）裂解酶活性两种活性。 N-糖基化酶活性能从双链DNA 上去除受损嘌呤碱基产生脱嘌呤 (AP) 位点，而 AP 裂解酶活性则能从 AP 位点的 3 处进行切割产生一个 5 磷酸和一个 3 磷酸-α，β-不饱和醛。 此外OGG1还能切割其它异常碱基对，比如与胞嘧啶配对的7,8-二羟基-8-氧代鸟嘌呤 (8-氧代鸟嘌呤), 与胞嘧啶配对的8-羟基 …

2018年5月24日 · Results showed that OGG1-BER further increases the levels of ROS-induced DNA damage by generating repair intermediates, leading to PARP1 overactivation and cell death.

2021年2月10日 · Our findings demonstrate that OGG1 is required to protect telomeres from OS and present OGG1 inhibitors as a tool to induce oxidative DNA damage at telomeres, with the potential for developing...

2019年2月1日 · Cytokine-induced ROS temporarily transform OGG1 into an epigenetic regulator of inflammatory gene expression with substantial impact on the cytokines that control lung cancer. In contrast to any attempt for a systemic intervention on ROS signaling with antioxidants, the disruption of NF-κB recruitment by stalled OGG1 is selective for the ...

2023年2月6日 · In this study we show Ogg1 -targeting siRNA mitigates bleomycin-induced pulmonary fibrosis in male mice, highlighting OGG1 as a tractable target in lung fibrosis. The small molecule OGG1...

OGG1 modulates the transcription of NF-κB target genes. Enzymatically inactive OGG1 (OGG1-SOH) by ROS binds to 8-oxoG located in gene regulatory regions and induces allosteric alteration of DNA, which facilitates NF-κB occupancy and the assembly of …

2024年6月1日 · ROS-inducing agents in combination with MTH1 inhibitors promote tumor cell parthanatos via OGG1-BER. A small amount of 8-oxoG integrated in the genome caused by lower doses of ROS-inducing agents can be cleared by OGG1-BER to maintain genome integrity.

2023年9月29日 · Blockade of OGG1 binding to DNA also prevented promoter recruitment of RelA/p65, Pol II, and p-RNAP II in a gene-specific manner. Collectively, the data presented offer new insights into how ROS signaling dictates NF-κB phosphorylation codes and how the promoter-situated substrate-bound OGG1 is exploited by aerobic mammalian cells for timely ...

4 天之前 · Oxidative stress, resulting from an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays a crucial role in tumor development. Tumor cells often experience elevated oxidative stress due to rapid proliferation and unstable metabolism, leading to DNA damage. The enzyme 8-oxoguanine DNA glycosidase (OGG1) is central to repairing oxidative DNA damage, thereby ...

Oxidatively-induced DNA damage and its repair as a transcriptional modulator. Reactive oxygen species (ROS)-induced DNA damage. The examples described above establish two key fundamental mechanisms through which cells use residual DNA damage and repair intermediates to activate (and suppress) cellular processes.