The opsonophagocytic killing assay (OPKA) is a critical tool for linking alterations in bacterial structure or function to subsequent changes in immune response and function. As such, it is frequently used as a complementary assay to determine immune-based efficacy of antibody treatments, vaccine candidates, enzyme optimization, etc.

The opsonophagocytic killing (OPK) assay is used as a correlate for protection to measure the functional capacities of vaccine-candidate-raised antibodies. This in vitro assay aids selecting promising vaccines by demonstrating whether the vaccine-induced antibodies drive efficient complement deposit …

2019年4月5日 · An opsonophagocytic killing assay (OPKA) is an experimental procedure in which phagocytic cells are co-cultured with bacterial units. The immune cells will phagocytose and kill the bacterial cultures in a complement-dependent manner.

What is an OPK assay? OPK assays play a key role in developing and improving vaccines, particularly pneumococcal vaccines. They are a useful addition to the pneumococcal antibody enzyme-linked immunosorbent assay (ELISA). The assay is used to quantify opsonic antibody activity present in blood serum samples.

2013年10月22日 · The opsonophagocytic killing (OPK) assay is used as a correlate for protection to measure the functional capacities of vaccine-candidate-raised antibodies. This in vitro assay aids selecting promising vaccines by demonstrating whether the vaccine-induced antibodies...

In fact, one measure of vaccine efficacy is the opsonophagocytosis killing (OPK) assay, which involves the engulfment of S. pneumoniae by human neutrophils in the presence of serum from a vaccinated individual. Antibodies also coordinate opsonophagocytosis in the clearance of H. influenzae and S. Typhi.

2018年11月7日 · Maximum rates of opsonophagocytic killing (OPK) of 14 different M types promoted by rabbit antiserum against the 30-valent M protein-based vaccine were comparable in whole-blood and HL-60 assays. Data are also presented showing OPK serum titers (opsonic index) of naturally acquired human antibodies present in IVIG [intravenous immune globulin ...

The opsonization assay has become an important tool in assessing pneumococcal vaccine immunogenicity (reference 1). To facilitate the widespread use of the pneumococcal opsonization assay, we developed and prepared a detailed protocol for a multiplexed opsonophagocytic killing assay (UAB-MOPA, reference 2). With minor modifications of this

The opsonophagocytic killing (OPK) assay is used as a correlate for protection to measure the functional capacities of vaccine-candidate-raised antibodies. This in vitro assay aids selecting promising vaccines by demonstrating whether the vaccine-induced antibodies drive efficient complement deposition and subsequent opsonophagocytic killing.

Pneumococcal vaccine development is driven by the achievement of high activity in a single gatekeeper assay: the bacterial opsonophagocytic killing (OPK) assay. New evidence challenges the dogma that anti-capsular antibodies have only a single function that predicts success.