Bacterial transformation plasmid for regulated expression of GFP. Sequence Author: Bio-Rad. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Efficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different BsmBI sites may not be compatible.

Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

Plasmid pGLO-GFP-3UAG from Dr. Chang Liu's lab contains the insert Green Fluorescence Protein with 3 UAG codons and is published in Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j. Epub 2015 Nov 27. This plasmid is available through Addgene.

2007年6月29日 · Neisseria gonorrhoeae expresses an O -linked protein glycosylation pathway that targets PilE, the major pilin subunit protein of the Type IV pilus colonization factor. Efforts to define glycan structure and thus the functions of pilin glycosylation (Pgl) components at the molecular level have been hindered by the lack of sensitive methodologies.

Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. Addgene has sequenced portions of this plasmid for verification. The results are shown below.

Bacterial transformation plasmid for regulated expression of GFP. How was your experience today? Addgene is a nonprofit plasmid repository.

2025年3月12日 · Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies. We have an expert-written solution to this problem! What is meant by control plate? What purpose does a control serve?

Lab report on E. Coli transformation using pGLO, detailing methods, results, and transformation efficiency. High school biology experiment.

2018年6月1日 · ORF 克隆是指从样本组织或细胞中抽提总 RNA，反转录，设计合成特异性引物后，通过 RT-PCR 扩增获得目的基因的 DNA 序列，获得编码全长蛋白质序列的 DNA 序列：即从起始密码 ATG 到终止密码子，不含 5′或 3′UTRs 非翻译区）及内含子序列。 技术应用. 目的基因的表达研究. 基因、蛋白的功能研究. 实验流程. 实验结果展示. A B C. 图1 目的基因的ORF克隆实验结果. A:样本1和样本2的RNA抽提电泳结果 ；B 不同扩增条件下目的片段的PCR扩增结果；C 目的 …