To better understand how EBNA1 controls these processes, we have assessed the contribution of various EBNA1 sequences to its replication, segregation, and transactivation functions. Here we show that EBNA1 residues 325 to 376 are responsible for the transactivation activity of EBNA1.

Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA1) is an essential viral protein and is expressed in all EBV-associated tumours as well as all latency programs of the virus, except perhaps for latency 0. It exerts essential functions in viral DNA replication and the episomal genome maintenance, achieved through sequence-specific DNA binding.

Assays for EBV serostatus assess antibody to both EBV viral capsid antigen (VCA) and Epstein-Barr nuclear antigen-1 (EBNA-1), but PTLD risk among SOT recipients with discordant VCA and EBNA-1 results is unknown.

2018年4月6日 · EBNA1 is essential for viral genome maintenance and also for controlling viral gene expression and without EBNA1, the virus cannot persist. EBNA1 itself has been linked to cell transformation but the underlying mechanism of its oncogenic activity has been unclear.

2024年10月10日 · In this proof-of-concept study, we employed a structure-based design strategy to create three unique immunogens specifically targeting the DNA binding state of the EBNA1 DBD. By immunizing mice, we successfully generated a mAb, named 5E2-12, which selectively targets the DNA binding interface of EBNA1.

We show that EBNA-1 induces chromosomal aberrations, DNA double-strand breaks, and engagement of the DNA damage response (DDR). These signs of genomic instability are associated with the production of reactive oxygen species (ROS) and are reversed by …

2020年3月19日 · 在一般的SN2亲核取代中，做为离去基团Br，I，Cl，OMs，OTf，OTs之间离去能力的比较？

2021年2月3日 · 近日，来自美国费城威斯达研究所的 Paul M. Lieberman 研究组在 Cell 杂志上发表了一篇题为 Cell-cycle-dependent EBNA1-DNA crosslinking promotes replication termination at oriP and viral episome maintenance 的文章， 揭示了EBNA1通过与DNA交联促进在oriP位点的复制终止实现附加体维持的具体分子机制。 EB病毒长期潜伏感染，会增加癌症和自身免疫疾病的风险 【1-3】。 EBNA1是潜伏感染期间病毒DNA复制和附加体维持所需的唯一病毒蛋白【4】。

EBNA-1是EBV潜伏感染时编码的一种核蛋白,它能调节EBV基因的复制,增强病毒基因的转录,并能保证EBV以附加体的形式稳定存在于被感染的细胞内。 利用此蛋白上述两种功能特点,研究者构建了EBV复制子载体,这种载体已被广泛地应用于基因治疗。 百度学术集成海量学术资源，融合人工智能、深度学习、大数据分析等技术，为科研工作者提供全面快捷的学术服务。 在这里我们保持学习的态度，不忘初心，砥砺前行。

Here, we provide a general overview of the EBNA3 genes and proteins. In particular, we describe recent research that has highlighted the complexity of their functional interactions with each other, with specific sites on the human genome and with the molecular machinery that controls transcription and epigenetic states of diverse host genes.