2020年6月16日 · Programmed ribosomal frameshifting (PRF) is the controlled slippage of the translating ribosome to an alternative frame. This process is widely employed by human...

2021年11月30日 · PRF 信号是嵌入在 mRNAs 中的顺式作用元件，随机地将核糖体翻译到一个新的阅读框中（即 +1 或 − 1 核苷酸）。 在典型的病毒环境中，PRF 信号允许核糖体绕过框终止密码子中编码的 0-框，继续合成 C-末端延伸的融合蛋白。

2019年5月10日 · Ornithine decarboxylase (ODC), a key enzyme in this process, is degraded by ornithine decarboxylase antizyme (OAZ). Synthesis of OAZ requires a +1 PRF event that is stimulated by polyamines. Thus, when polyamine levels are low, OAZ is not made, and ODC is available to synthesize polyamines.

2024年2月1日 · Down-regulation of the titration of OAZ via AZIN leads to enhanced degradation of OAZ-targeted ODC, reducing ODC, inhibiting putrescine synthesis, and suppressing further +1 PRF. In addition, eIF5A depletion also leads to decreased translation of HOL1 mRNA, reducing the levels of polyamines and repressing the +1 PRF.

2016年2月19日 · Programmed −1 ribosomal frameshifting (−1 PRF) has been identified as a mechanism to regulate the expression of many viral genes and some cellular genes. The slippery site of...

2011年9月7日 · Ornithine decarboxylase (ODC), the rate-limiting enzyme in the biosynthesis of polyamines, is regulated by an antizyme (OAZ). Polyamines induce antizyme expression by promoting...

2018年4月2日 · OAZ +1 PRF is autoregulated by the availability of small molecules in the form of polyamines (i.e., the products of the synthetic pathway controlled by OAZ). OAZ downregulates polyamine synthesis by stimulating ubiquitin-independent degradation of ornithine decarboxylase (ODC), the enzyme that catalyzes the first step in polyamine biosynthesis.

The results showed that eIF5A is a trans-regulator of +1 PRF for Ty1 retrotransposon and could function universally in yeast. View PRF at the shifty site derived from the OAZ1 in S. cerevisiae...

2014年1月1日 · Polyamine-regulated synthesis of OAZ involves a ribosomal frameshifting event at an internal STOP codon of OAZ mRNA. OAZ forms inactive ODC/OAZ heterodimers, in which ODC exposes an unstructured domain termed ODC degradation signal (ODS). Exposure of ODS by OAZ leads to ubiquitin-independent degradation of ODC by the 26S proteasome.

2023年12月15日 · Our results show the expanded function of the CRISPR-Cas12 system in modulating −1 PRF efficiency through stalling ribosomes and deforming frameshifting stimulatory signals, which could serve as a new strategy for future coronavirus pandemics.