
Oxygen glucose deprivation/re-oxygenation-induced neuronal …
2020年9月30日 · Notably, the OGD/R-induced decrease in viability (CCK-8 OD) (Fig. 2b) and cell death (LDH medium release, Fig. 2c) were significantly attenuated by miR-422a inhibition.
The protection impact of tectoridin on PC12 cell preventing OGD…
2023年2月15日 · Tectoridin improved cell viability and reduced the release of LDH damaged by OGD/R. A. Chemistry-based structure exhibited by tectoridin. B. PC12 cell was cultured by …
丹参酮IIA减轻氧糖剥夺诱导心肌细胞损伤的机制 - PMC
1.2.5. 细胞培养液中LDH水平的检测. 采用分光光度法检测细胞培养液中LDH的水平以反映细胞中LDH的漏出率。收集各组细胞的培养液,按照LDH检测试剂盒说明书,使用酶标仪测定450 …
The Duration of Oxygen and Glucose Deprivation (OGD) …
Reperfusion following shorter periods (4 and 6 h) of OGD recovered apoptotic PC12 cells, whereas after 12 h, OGD increased LDH release. In primary neurons, 6 h OGD led to …
Stretch and/or oxygen glucose deprivation (OGD) in an
2015年8月21日 · LDH generated after stretch, OGD, a combination of both and successive reoxygenation was measured. LDH was expressed as a percent of the total releasable LDH …
Lactate Protects Microglia and Neurons from Oxygen–Glucose
LDH inhibition reduced cell survival and the extracellular levels of lactate in either normoxia or OGD-exposed cells, possibly due to the LDH committals in NAD + recycling, which may have …
肌肽通过激活Nrf2/ARE信号通路改善OGD/R诱导星形胶质细胞损伤 …
Astrocytes were randomly divided into control group, OGD/R group, and OGD/R+carnosine group (1, 3, 9 mmol/L). The morphological changes of cells were observed by inverted microscope. …
【求助】氧糖剥夺/再灌注(OGD/R) - 丁香通研选
2023年5月5日 · 乳酸脱氢酶(ldh)释放实验是另一种常用的细胞毒性筛选方法。这种方法是通过检测培养液中乳酸脱氢酶的活性来评估细胞膜损伤程度,进而反映细胞死亡率。您可以尝试使 …
Melatonin reduces OGD/R-induced neuron injury by regulating …
Results: Melatonin enhanced the viability and reduced the death and LDH release of OGD/R exposed SH SY5Y cells. Melatonin repressed the HMGB1, TNF-α, and iNOS mRNA …
The protective effect of dopamine against OGD/R injury …
2016年3月1日 · OGD performed for 18 h was followed by re-oxygenation for 10 and 20 h to determine the optimum period of re-oxygenation. Cells cultured under normal conditions …