The present study aimed to explore the mechanisms of action of long non-coding RNA (lncRNA) maternally expressed gene 3 (Meg3) in angiogenesis following OGD. The human brain microvascular endothelial cell line, hCMEC/D3, was used to establish the OGD model. lncRNA Meg3 was highly expressed in hCMEC/D3 cells subjected to OGD.

2019年3月26日 · In this study, we investigated the cellular response of two iPSC-derived brain microvascular endothelial cell (BMEC) monolayers to respond to oxygen-glucose deprivation (OGD) stress, using two induced pluripotent stem cells (iPSC) lines. iPSC-derived BMECs responded to prolonged (24 h) and acute (6 h) OGD by showing a decrease in the barrier ...

2010年5月21日 · These data show that neutrophils can inhibit permeability increases associated with 1 h of exposure to OGD in the hCMEC/D3 model. This is mimicked by application of neutrophil supernatants and in the presence of antiadhesion …

氧糖剥夺/再灌注（Oxygen Glucose Deprivation/Reperfusion, OGD/R）细胞模型是研究缺血性脑卒中等疾病的重要工具，它通过模拟体内细胞在缺血缺氧后再灌注的损伤过程，为疾病机制和药物筛选研究提供了有力的实验平…

Conversely, stress conditions and extracellular stimuli have been shown to increase paracellular permeability of hCMEC/D3 cells via signaling pathways such as JNK, PKC or NFκB. These include mannitol treatment, oxygen and glucose deprivation (OGD) and pro-inflammatory cytokines such as TNFα and chemokines such as CCL2.

2025年4月1日 · Our study reveals that HDI-1 expedites the restoration of tight junction proteins, Occludin and Claudin-5, in the oxygen-glucose deprivation/reoxygenation (OGD/R) model using human cerebral microvascular endothelial cells (hCMEC/D3). Moreover, HDI-1 mitigates the impairment of cellular monolayer membrane permeability following injury.

2023年10月3日 · Transcriptomic and proteomic analyses were performed on human cerebral microvascular endothelial cells following oxygen–glucose deprivation (OGD) or OGD plus recovery (OGD/R) to identify molecules and signaling pathways dysregulated by reperfusion. Major findings were further validated in a mouse model of cerebral ischemia and reperfusion.

2022年8月11日 · The present study aimed to explore the mechanisms of action of long non-coding RNA (lncRNA) maternally expressed gene 3 (Meg3) in angiogenesis following OGD. The human brain microvascular endothelial cell line, hCMEC/D3, was used to establish the OGD model. lncRNA Meg3 was highly expressed in hCMEC/D3 cells subjected to OGD.

2024年1月9日 · After interfering with FOXO4 in cells, the viability of the OGD/R-induced HCMEC/D3 cells was increased while apoptosis was decreased. Furthermore, FOXO4 interference improved cellular barrier dysfunction but inhibited oxidative stress and the inflammatory response in HCMEC/D3 cells induced by OGD/R. FOXO4 knockdown regulated CTRP6 transcription ...

2017年5月15日 · For this reason, we investigated the potential alterations of antioxidant responses in the cerebral endothelium, exposing hCMEC/D3 cells to two different treatment (DEP or OGD) and to a combined treatment (DEP followed by OGD).