Here, we present a protocol for the generation and metabolic characterization of high-fat diet (HFD)-fed mice as a frequently used model of diet-induced insulin resistance. We illustrate in detail the oral glucose tolerance test (OGTT), which monitors the peripheral disposal of an orally administered glucose load and insulin secretion over time.

2022年1月17日 · In this study, AL- and HFD-treated male wild-type mice at 20 weeks of age clearly showed higher IGT by OGTT than the other two HFD-treated groups. AL reportedly destroys pancreatic β-cells...

It found that mouse strain may impact on the body weight increment, and weight ratio of adipose tissue. All four HFD treated mouse strains showed significant lipid accumulation in liver and adipose tissue. OGTT and ITT indicated that all four obese mouse strains response to glucose and insulin load in similar manner.

我们详细说明了口服葡萄糖耐量试验（OGTT），该试验监测随时间推移口服给药的葡萄糖负荷和胰岛素分泌的周边情况。 此外，我们提出了胰岛素耐受性测试（ITT）的协议，以监测全身胰岛素的作用。 一起，这些方法及其下游应用代表了表征小鼠一般代谢表型以及特异性评估葡萄糖代谢变化的强大工具。 它们在胰岛素抵抗，糖尿病和肥胖症的广泛研究领域中可能特别有用，以提供对发病机理的更好理解以及测试治疗干预的效果。 Study of In Vivo Glucose Metabolism in High …

2020年11月8日 · 如果是通过口服给予葡萄糖，就是 口服糖耐量试验（「oral glucose tolerance test」）简称为： 「OGTT」；如果是通过腹腔注射来给予葡萄糖，就是 经腹腔注射葡萄糖耐量检测（「intraperitoneal glucose tolerance test」）简称为 「IPGTT」如果葡萄糖耐量降低，机体消耗葡萄糖的能力下降，在外源给予葡萄糖之后，血糖相对迅速上升而下降缓慢。 在临床上，这个实验也十分普遍，当然，临床中是通过口服葡萄糖来实现的，这个试验也是临床诊断糖尿病的金 …

2025年1月4日 · At the end of 15th week post-treatment, we performed an OGTT. HFD + L-NAME treatment elevated fasting glucose levels (Fig. 3 A) and resulted in higher glucose levels during OGTT in mice (Fig. 3 B). Nitazoxanide and dapagliflozin treatments significantly improved HFD + L-NAME-induced glucose handling impairment in mice (Fig. 3 A–C).

An oral glucose tolerance test (OGTT), in which the mice are challenged with a bolus of glucose and blood glucose and insulin levels are measured across a two hour time course is performed one week prior to the initiation of the HFD challenge. The mice are subjected to a seven-week HFD challenge using a Western diet.

2021年12月1日 · Our aims were to explore and establish an optimized induced T2DM model in mice that exhibits insulin resistance and β-cell damage. C57BL/6 mice were treated with a high-fat diet (HFD), streptozotocin (STZ) and dexamethasone (DEX) at …

Glucose tolerance was most different and therefore significant (P = 0.001) in high-fat-fed mice after 6 h of fasting (1,973 +/- 96 vs. 1,248 +/- 83 mmol.l (-1).120 min (-1)). The difference in glucose tolerance was greater following an OGTT (142%), in contrast to an IPGTT, with a 127% difference between high fat and chow.

2015年6月19日 · Under the dynamic physiologically relevant conditions of the OGTT, skeletal muscle from HFD fed mice exhibits alterations in glucose metabolism at the level of the TCA cycle. Defects in muscle glucose metabolism, particularly insulin resistance, are a characteristic feature of obesity and type 2 diabetes [1].