As mentioned above, HRMS is an attractive platform for the analysis of oligonucleotides as it is well suited towards sensitive bioanalysis and enables the ability to detect and quantify impurities/metabolites within the sample. Additionally the full scan MRMHR data can be assessed to gain an understanding of

Jul 5, 2021 · In this study, we developed a generic HILIC hyphenated with high-resolution mass spectrometric (HILIC−HRMS) method to provide rapid, robust, and in-depth analytical characterization of DNA/RNA oligonucleotides.

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Dec 29, 2023 · Herein, we present a direct method employing fully resolved isotopic envelopes, enabled by high resolution mass spectrometry (HRMS), to identify and quantify isobaric impurity ions resulting from the deletion or addition of a uracil (U) or cytosine (C) nucleotide from or to the full-length sequence.

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HRMS enables characterization (identifying different isotopes/charge states) of an oligonucleotide molecule and the ability to select isotope/charge state best suited to achieve good sensitivity, selectivity, and accuracy for quantitation.

Jul 5, 2021 · High-resolution mass spectrometric (HRMS) analysis was used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities. To enable unbiased sequence characterization with tandem mass spectrometry (MS/MS), we also optimized higher-energy C-trap dissociation (HCD) on improving the sequence coverage of DNA and ...

•Synthetic oligonucleotides, as an evolving class of therapeutics, pose unique scientific and regulatory challenges. •In-depth impurity assessment offers comprehensive impurity

Results & methodology: HPLC coupled with high-resolution mass spectrometry (LC-HRMS) methods were developed to investigate the metabolism of a REVERSIR™ molecule in vivo. Plasma and tissue samples were extracted using solid-phase extraction followed by LC-HRMS analysis for metabolite profiling and quantification.

In the area of oligonucleotides, HRMS in particular has been increasingly utilized.1 In this application note, we outline the performance capabilities of the Xevo G2-XS QTof HRMS platform for oligonucleotides as well as compare typical bioanalytical figures of merit to the triple quadrupole platforms. Xevo