The OME-TIFF file format can support any image organization. However, using one TIFF file per timepoint per channel with the focal planes for that timepoint and channel stored sequentially within the TIFF makes for very easy creation of TiffData elements (see 5D datasets ).

OME data model specification, code generator and implementation. To contribute to the OME Model documentation, go to the documentation repository. We welcome pull requests from anyone, but ask that you please verify the following before submitting a pull request:

This section provides some sample data in OME-TIFF format. They include data produced from an acquisition system as well as artificial sample datasets, i.e. designed for developer testing that illustrate some possible data organizations, which should be useful if you are interested in implementing support for OME-TIFF within your software.

OME-TIFF supports multi-resolution images or pyramidal images where individual planes are stored at different levels of resolution. The downsampled image planes are called pyramidal levels, sub-resolution image planes or sub-resolutions. Supported resolutions OME-TIFF planes can be reduced along the X and Y dimensions.

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From ome-model 6.0.0, the OME-TIFF file format specification includes support for multi-resolution images - see the OME-TIFF specification and the Bio-Formats 6.0.0 announcement. Bio-Formats and OME-Files API and implementation changes

This documentation covers the OME Data Model, OME-XML and OME-TIFF. The OME Model is a specification for storing data on biological imaging. The model includes image parameters, such as XYZ dimensions and pixels type, as well as extensive metadata on, for example, image acquisition, annotation, and regions of interest (ROIs).

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The OME-XML file serves as a convenient file format for data migration from one site or user to another. The OME-XML file captures all image acquisition and experimental metadata, along with the binary image data, and packages it into an easily readable package.

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