磷酸化修饰 （Phosphorylation modification），目前研究最为广泛的PTMs，是原核生物和真核生物中最重要的调控修饰形式。 因其调控底物蛋白活性的特点，磷酸化修饰被称为 蛋白质的功能“开关”，涉及几乎所有细胞过程中蛋白质在时间和空间尺度上的调节，如蛋白质活性、稳定性、构象、与其他生物分子的互作等，影响着包括细胞代谢、生长、分裂、分化、运动、细胞器运输、膜运输、肌肉收缩、免疫、学习和记忆等在内的每一个基本生命过程。 蛋白磷酸化是指激酶催化γ-磷 …

Posttranslational modifications (PTMs) are covalent processing events that change the properties of a protein by proteolytic cleavage and adding a modifying group, such as acetyl, phosphoryl, glycosyl and methyl, to one or more amino acids .

In molecular biology, post-translational modification (PTM) is the covalent process of changing proteins following protein biosynthesis. PTMs may involve enzymes or occur spontaneously. Proteins are created by ribosomes, which translate mRNA into polypeptide chains, which may then change to form the mature protein product.

PTMs通常是指在蛋白质生物合成过程中或之后对折叠或新生蛋白质进行共价和酶促改变 [2]，从而改变蛋白质的性质及功能。 蛋白质的PTMs方式多样，已经确定的修饰类型超过500种，以 磷酸化 、 泛素化 、 甲基化 、 乙酰化 、 糖基化 等比较常见，一般会使蛋白质的氨基酸残基发生固定的分子质量偏移（表1）；报道的PTMs生物学功能包括细胞定位、蛋白质的翻转和活性状态、基因表达的调节、酶的激活、相互作用等 [3]。 表1 常见的蛋白质PTMs. 基于 生物质谱 的蛋白质组学 …

In this work, we reported PTMD, a well-curated database containing PTMs that are associated with human diseases. We manually collected 1950 known PDAs in 749 proteins for 23 types of PTMs and 275 types of diseases from the literature.

Recent advances in mass spectrometry (MS)-based proteomics allow the identification and quantitation of thousands of posttranslational modification (PTM) sites in a single experiment. This follows from the development of more effective class ...

The PTM substrate sites locating in a specified distance of the amino acids encoded from nsSNPs were referred to having an association with its involving diseases (Figure 1). In recent years, an increasing evidence for crosstalk between PTMs has been reported.

2018年5月22日 · We coupled immunoprecipitation using PTM-specific antibodies with tandem mass tag (TMT) mass spectrometry to simultaneously examine phosphorylation, methylation, and acetylation in 45 lung cancer cell lines compared to normal lung tissue and to cell lines treated with anticancer drugs.

2018年5月18日 · Reversible post-translational modification (PTM) is a powerful and ubiquitous mechanism to regulate protein function. The mechanistic basis of the associated functional regulation by PTMs often involves the recruitment of interaction partners that selectively bind the modified protein.

2025年1月8日 · Here, we present PTMNavigator, a tool integrated into the ProteomicsDB platform that offers an interactive interface for researchers to overlay experimental PTM data with pathway diagrams....