2021年11月3日 · In overcrowded HSQC spectra, proton multiplet structures of cross peaks set a limit to the power of resolution and make a straightforward assignment difficult. In this work, we provide a solution...

Spectral overlay shows chemical shift changes in the RAS-like domain relative to that of the WT. Arrows indicate examples of shifted peaks in the spectrum of QP in putative nucleotidebinding ...

2025年3月17日 · The following list are a few advantages for overlaying (also referred to as collect or dual mode) an HSQC with an HMBC: HMBC correlations can be used to differentiate overlapping HSQC correlations (and vice versa), by lining-up the correlations for both experiments, fragments can be quickly pieced together,

2018年1月11日 · In case of 13 C chemical shifts overlap, 1 H chemical shifts can be used to achieve the desired resolution. The proposed (3, 2)D 1 H, 13 C BIRD r,X -HSQC-TOCSY experiment achieves this while preserving singlet character of cross peaks in the F1 dimension.

2023年1月1日 · In this chapter, we highlight the potential of a class of methods called intrinsically quantitative 2D HSQC. They are generated by designing pulse sequences able for each correlation peak to maintain constant or to compensate JHH, JCH, off resonances and T2 effects responsible for the degradation of the quantitativity.

(a) Overlay of 2D [1H, 15N] Heteronuclear Single-Quantum Correlation (HSQC) spectra of 15N-labeled b2m 45 mM in the free form in blue and in the presence of peptide 461 in red (peptide/protein...

2020年8月24日 · The toolbox of NMR methods for isotopomer analysis includes acquisition techniques such as 2D-HSQC and HMBC NMR experiments, which correlate proton frequencies with the relevant heteronucleus (usually 13 C or 15 N in MFA experiments), and the HSQC-TOCSY NMR experiment, which can be used to identify molecules with multiple labels.

Make some assignments of your compound based on COSY, TOCSY and HSQC data. Look in particular for entry points into the spectra, some proton and/or carbon that can be assigned with definiteness that show correlations in the COSY/TOCSY/HSQC spectra.

Figure 2 shows an overlay of the HSQC and HSQC-TOCSY spectra. From the interpretation of the HSQC spectrum it is evident that the sample consists mainly of a major isomer of C 2 symmetry...

2010年1月5日 · Topspin also has a nice feature for overlaying 1D spectra. Simply click the overlay icon and enter the spectral overlay mode. Then drag the desired experiment into the spectral window. Various features of either spectrum (i.e., vertical position, data height, spectral width) can be changed using the icons in this mode.