Monoclonal Antibody for studying Smad1 (Ser463/Ser465) phosphate/Smad5 (Ser463/Ser465) phosphate/Smad9 (Ser465/Ser467) phosphate. Validated for ChIP, F, IF, IP, WB. Available in 2 sizes.

Phospho-SMAD/5 (Ser463/465) (41D10) Rabbit mAb detects endogenous levels of SMAD1 and SMAD5 only when dually phosphorylated at Ser463 and Ser465 and is also predicted to detect SMAD9 (SMAD8) when phosphorylated at Ser465 and Ser467. The antibody does not cross-react with other SMAD-related proteins.

Smad（由线虫的Sma和果蝇的Mad缩写而来）蛋白是TGF-β家族受体下游的信号转导分子。在哺乳动物中总共存在八个Smad蛋白（Smad1到Smad8）。 根据在TGF-β信号转导中的功能差异，它们被分为三类： ① 包括受体调控的Smad（receptor-regulated Smad， R-Smad – Smad1/2/3/5/8）

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemistry and Western Blotting. Key Applications: Western Blotting; Immunohistochemistry (Paraffin) Application Notes

Invitrogen Anti-Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465) Polyclonal, Catalog # PA5-64711. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

Smad蛋白（Smad是由线虫的Sma和果蝇的Mad缩写而来） 是TGF-β家族受体下游的信号转导分子[1,2]。在哺乳动物中有八个Smad蛋白, Smad1、 Smad1、 ...、 Smad8。 根据在TGF-β信号转导中的功能差异，它们被分为三类： 与其它跟许多激酶 - 底物反应一样， TGF- β I 型受体对 R-Smad 的磷酸化需要它们的直接结合。

Invitrogen Anti-Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Monoclonal (31H14L11), Catalog # 700047. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human samples. Supplied as 100 µg purified antibody (0.5 mg/mL).

Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemistry and Western Blotting.

Smad是某些基因的 转录激活因子，例如活性Smad（白细胞抑制因子） 启动子 近端的Smad-binding element（Smad结合元件， SBE）结合。 当Smad4或Smad2/3被 siRNA 干扰时， TGF -β诱导LIF表达的作用下降了。 Smad2或3只敲除一个，则LIF表达量仍较高。 Smads家族蛋白在将TGF-β信号从细胞表面受体传导至细胞核的过程中起到关键性作用，且不同的Smad介导不同的TGF-β家族成员的信号转导。 TGF-β作为配体形成的受体复合物，激活Smads进入核内，共同 …

P-Smad1/5/8 is a laboratory reagent used to detect phosphorylated Smad1, Smad5, and Smad8 proteins. These Smad proteins are key mediators of the BMP signaling pathway. This product can be used in techniques such as Western blotting to analyze the activation status of the BMP signaling cascade.