2014年9月15日 · Here we describe a technique first introduced by Passini and Wolfe for direct intracranial delivery of virally-encoded transgenes into the neonatal mouse brain. In its most basic form, the procedure requires only an ice bucket and a microliter syringe.

2021年2月8日 · ICV injections were used to deliver AAV9-sgRNAs directly into the CSF of neonatal mice at postnatal day 0 (P0) to enable widespread transduction in the CNS [36, 37].

2020年1月14日 · These data indicated that, in contrast with P0 ICV injection, intravenous injection of PHP.eB AAV in adult mice results in robust base editing in unsorted cerebellar tissue.

Herein, we describe a fast, easy, and economical method for widespread neuronal manipulation via intraventricular injection of adeno-associated virus (AAV) into the neonatal mouse brain.

2024年1月16日 · A pup injection with the transgene-expressing AAV9 using stereotaxic ICV coordinates at P0. B Molecular and cellular approaches to validate the gene expression in the adult mouse brain and spinal cord.

2017年12月15日 · Each AAV was administered to P0-neonatal mice via intracerebroventricular injections (ICV). Brains were then systematically analyzed for GFP expression at 3 or 6 weeks post-infection in various regions, including the olfactory bulb, striatum, cortex, hippocampus, substantia nigra (SN) and cerebellum.

2017年12月15日 · Each AAV was administered to P0-neonatal mice via intracerebroventricular injections (ICV). Brains were then systematically analyzed for GFP expression at 3 or 6 weeks post-infection in various regions, including the olfactory bulb, striatum, cortex, hippocampus, substantia nigra (SN) and cerebellum.

2023年5月4日 · When assessed in the brain by P0 ICV administration of 1 × 10 11 vg of v1 PE3-AAV9, epegRNAs more strongly increased Dnmt1 +1 C-to-G editing efficiencies over pegRNAs from 6.1% to 21% prime ...

2014年9月15日 · Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

Each AAV was administered to P0-neonatal mice via intracerebroventricular injections (ICV). Brains were then systematically analyzed for GFP expression at 3 or 6 weeks post-infection in various regions, including the olfactory bulb, striatum, cortex, hippocampus, substantia nigra (SN) and cerebellum.