通过雌性p16-InkAttac小鼠p16 阳性衰老细胞清除实验，发现衰老指标部分恢复到年轻水平，揭示了衰老过程中大脑免疫细胞宏观的动态重塑，提示将 靶向衰老细胞 作为对抗炎症变化和认知能力下降的策略。

2024年11月11日 · Our data demonstrate that CSS induces a p16Ink4a -dependent increase in key senescence markers in select organs in male mice, while CRS increases p21 Cip1 exclusively in the brain of male but not...

To address the relationship between senescent cell accumulation and brain aging, we designed studies to eliminate highly p16 Ink4a ‐expressing cells through pharmacogenetic (INK‐ATTAC mouse model) and senescent cells through pharmacologic (senolytic drugs: Dasatinib and Quercetin (D + Q)) approaches. We hypothesized that removal of ...

We examined expression of senescence (p16 and p21) and DNA damage markers (8-OHdG and γH2AX) in motor cortex (MCx), frontal association cortex (FACx) and occipital cortex (OCx) in post-mortem tissue donated by patients with ALS/MND and controls.

2024年3月16日 · We found that p16 ink4a expression was increased in microglia near amyloid plaques in brain tissue from patients with AD and 5XFAD mice, a model of AD.

2023年6月23日 · We demonstrated that senescent cells accumulate in both the brain and spinal cord with age using p16 reporter (p16-luc) mice 38, which can help visualise senescent cells in vivo.

2024年10月4日 · Currently, p16 Ink4a is one of the widely used markers for studying cellular senescence, and it plays a prominent role in inhibiting CDK4/6 and restricting the progression of G1 to S phase in the cell cycle. 16, 17 Numerous studies have utilized the activation of the p16 Ink4a promoter to identify cellular senescence in vivo and have thus ...

2022年9月27日 · Activated resident and infiltrating immune cells increase in the aged brain and are partially restored to youthful levels through p16-positive senescent cell clearance in female p16-InkAttac...

Here we assessed the effect of chronic stress on cellular senescence-a hallmark of aging. Exposure to restraint stress, a psychological non-social stress model, increased p21 Cip1 exclusively in the brains of male, but not female mice, and in a p16 Ink4a -independent manner.

表现出衰老和疾病相关激活特征（包括趋化因子上调）的 p16 阳性髓系细胞在老年小鼠大脑中积累。 衰老的脑髓细胞在体外促进外周免疫细胞趋化性。 活化的驻留和浸润免疫细胞在老年大脑中增加，并通过雌性 p16-InkAttac 小鼠的 p16 阳性衰老细胞清除部分恢复到年轻水平，这与认知功能的保存有关。 我们的研究揭示了衰老过程中脑免疫细胞景观的动态重塑，并建议将衰老细胞靶向作为对抗炎症变化和认知能力下降的策略。 Cellular senescence is a plausible mediator of …