293细胞中转入 SV40T-antigen基因 形成的高转衍生株，即为293T细胞系。这使得包含SV40ori的质粒能够在该细胞系中显著扩增，从而促进表达载体的扩增，促进蛋白的表达。

2022年12月13日 · 摘要： 目的 探讨含WW结构域的E3泛素蛋白连接酶2 (WWP2)调控p21稳定性的潜在作用机制。 方法 通过免疫共沉淀及Western blotting检测HEK 293T细胞中WWP2与p21的结合情况。 用蛋白酶体抑制剂 (MG132)和放线菌酮 (CHX)处理过表达或敲减 WWP2 HEK 293T细胞0、4、8 h, 通过Western blotting检测p21的表达情况。 结果 WWP2与p21间存在结合。 过表达 WWP2 的293T细胞中, p21的表达水平明显降低, 且随WWP2表达量增加逐渐降低。 敲减 WWP2 …

2022年5月20日 · HEK-293人胚肾细胞：是用5型腺病毒75株系转化，含有Ad5 E1区的人胚肾亚三倍体细胞系，是一种E1区缺陷互补细胞系。 它是加拿大McMaster University 的F.L.Graham与J.S.Miley于1976年用DNA转染技术构建而成。 该细胞为人类腺病毒载体扩增的宿主。 可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 HEK-293T人胚肾细胞：人胚肾细胞293细胞插入SV40 T-抗原基因后产生的高转染效率的衍生株称为293T。 …

2022年9月17日 · To investigate the possible role of p21 in regulating the expression of endogenous p53 targets, we analyzed mRNA levels of Puma, Mdm2, and Gadd45a in untreated control and γ-ray-irradiated cells.

mouse Waf1/Cip1/CDKN1A p21 transfected 293T lysate; 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for Waf1/Cip1/CDKN1A p21 antibodies

2024年3月1日 · P21 is encoded by the CDKN1A gene, a classic target and effector of P53. P21 potently inhibits cyclin-dependent kinases (CDK) including CDK4/5, CDK2, and CDK1 (19). Through inhibiting CDKs, P21 can then activate another group of tumor suppressors, the RB family, which would otherwise be phosphorylated and inactivated by CDKs (19).

2018年1月22日 · Human embryonic kidney 293T, human glioblastoma cell line U87, and human liver HepG2 cell lines were obtained from cell bank of Chinese Academy of Sciences (Shanghai, China). 293T and U87 cells were grown in Dulbecco’s modified Eagle’s medium (Wisent, Montreal, QC) supplemented with 10% fetal bovine serum (Wisent, Montreal, QC) and 1% ...

Here, we find that p21 levels are elevated in response to influenza A virus (IAV) infection, which is independent of p53. Silencing, pharmacological inhibition or deletion of p21 promotes virus...

Furthermore, we have also shown that overexpression of E2A in 293T cells activates expression of the endogenous p21 gene at both the levels of mRNA and protein. In correlation with the finding that E47 overexpression leads to growth arrest in NIH 3T3 cells, we have shown that Id1 overexpression in NIH 3T3 cells accelerates cell growth and ...

前期针对p21 基因启动子设计的saRNA（dsP21－322）能在前列腺癌、膀胱癌等细胞系中明显激活p21表达，并且上调的p21表现出相应的生物学活性。 本研究又证实dsP21－322在骨肉瘤及宫颈癌等实体肿瘤细胞系及正常的肾上皮细胞系中激活p21的表达，显示出RNAa现象在人类 ...