p22phox Protein, also known as the human neutrophil cytochrome b light chain (CYBA), is an essential component of the membrane-associated enzyme phagocyte NADPH-oxidase [1] This enzyme uses NADH or NADPH as the electron donor for the one electron reduction of oxygen to produce superoxide anion, a reactive oxygen species (ROS), [2] and a ...

P22phox is a ubiquitous protein encoded by the CYBA gene located on the long arm of chromosome 16 at position 24, containing six exons and spanning 8.5 kb. P22phox is a critical component of the superoxide-generating nicotinamide adenine ...

Here, we show that p22phox is associated with Rho-associated coiled-coil kinase 2 (ROCK2) in human monocytes but not neutrophils. This interaction occurs between the cytosolic region of p22phox (amino acids 132 to 195) and the coiled-coil region of ROCK2 (amino acids 400 to 967).

P22phox also called the α-subunit of the flavocytochrome b558, is composed of 195 amino acids and runs at 22 kDa on SDS-PAGE (Parkos et al., 1988). It has two or three predicted transmembrane α-helices and a C-terminal cytosolic tail which contains one proline rich region (PRR) (Pro156-Pro-Arg-Pro159) which binds to p47phox-src homology 3 ...

我们提供的第一个证据表明，p22（phox）是产生超氧化物的血管NADH / NADPH氧化酶的关键成分，并暗示该氧化酶系统在血管肥大中的重要作用。 超氧阴离子的形成对于吞噬细胞的杀微生物活性至关重要。 但是，最近有越来越多的证据表明它也参与血管平滑肌细胞（VSMC）的细胞生长。 我们已经表明，肥大剂血管紧张素II通过激活膜结合的NADH / NADPH氧化酶来刺激超氧化物的产生，并且对该氧化酶的抑制会减弱血管肥大。 但是，尚不清楚该氧化酶在VSMC中的分子身份 …

2015年9月29日 · Downregulating p22phox resulted in decreased ROS generation, a reduction of NOXs (NOX1, 2, 4) and a decrease in inflammatory cytokine. In addition, p22phox downregulation reduced the activation...

Using computer molecular modelling we discovered that C242T SNP causes significant structural changes in the extracellular loop of p22 phox and reduces its interaction stability with Nox2 subunit.

2024年10月13日 · The Nox subunit p22phox is essential for Nox1–4 activity, and p22phox and Nox2 proteins are mutually stabilized in phagocytic cells. This study investigated the suitability of p22phox protein as a marker of Nox activity.

2019年1月1日 · Aim: In this study, we investigated the role of p22phox, an essential component of most NADPH oxidases, in the UPR of endothelial cells. Results: Induction of ER stress increased p22phox expression at the transcriptional level. p22phox was identified as novel target of the UPR transcription factor ATF4 (activator of transcription factor 4 ...

2020年2月10日 · The C242T polymorphism in the p22PHOX gene encoding essential subunit of NADPH oxidase was associated with CVD, hypertension, and endothelial function. The aim of this study was to assess a ...