pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP - Addgene
Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation
Methylated PP2A stabilizes Gcn4 to enable a methionine
2020年12月25日 · Here methionine directs the conserved methyltransferase Ppm1 via its target phosphatase PP2A to selectively stabilize Gcn4. Through this, cells conditionally modify a …
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP 载体图谱和序列 - 纽普生物
提供 pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP 载体/质粒图谱，全长序列，抗性，大小和元件信息。
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP Sequence and Map
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 10 copies of an optimized GCN4_v4 peptide. Sequence …
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP载体_质粒图谱 - 优宝生物
Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 载体
dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system
A Protein-Tagging System for Signal Amplification in Gene Expression ...
2014年10月23日 · To distinguish between these possibilities, we inserted a viral P2A ribosome skipping sequence in between the 24xGCN4 peptide array and GFP, which allows synthesis of …
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE from Dr. Ron Vale's lab contains the insert dCas9 and is published in Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. …
dCas9-5xGCN4-P2A-BFP - Addgene
Plasmid dCas9-5xGCN4-P2A-BFP from Dr. Rhys Allan's lab contains the insert dCas9-5xGCN4 and is published in Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4. …
1.2 GCN4的结构和功能特点_酵母转录激活因子与DNA分子识别的 …
GCN4是碱性区-亮氨酸拉链（basic region-leucine zipper）模型中典型的一种。 与其他几种DNA结合蛋白结构域相比，bZIP结构域模型简单清晰，两个结构单元碱性区和拉链区分工明确，既相 …