2023年6月8日 · (G) Myh11 -CreNLS P2A KI mice were crossbred with ROSA26-driven mT/mG reporter mice. Cre activity in the aorta is proportional to the loss of tdTomato (red) signal and gain of EGFP (green) signal.

2023年6月8日 · We used CRISPR/Cas9-mediated homologous recombination between a donor vector carrying the CreNLS P2A or CreER T2–P2A sequence and homologous arm surrounding the translation start site of the Myh11 gene to generate Cre-knockin mice. The P2A sequence enables the simultaneous translation of Cre and endogenous proteins.

将P2A-Cre-T2A-tdTomato插入到小鼠Ddx4基因终止密码子处。 应用领域:Cre工具鼠,生殖系统*使用本品系发表的文献需注明: Ddx4-P2A-Cre-2A-tdTomato mice (Cat. NO. NM-KI-225028) were purchased from Shanghai Model Organisms Center, Inc..

The brief process is as follows: CRISPR-Cas9 system and donor were microinjected into the fertilized eggs of C57BL/6JGpt mice. Fertilized eggs were transplanted to obtain positive F0 mice which were confirmed by PCR and sequencing. A stable F1 generation mouse model was obtained by mating positive F0 generation mice with C57BL/6JGpt mice.

Both constitutive (Myh11 -CreNLS P2A) and inducible (Myh11 -CreER T2–P2A) Cre mice demonstrated efficient, SMC-specific, sex-independent Cre recombinase activity without confounding endogenous gene expression.

knock-in mice. The P2A sequence enables the simultaneous translation of Cre and endogenous proteins. Using reporter mice, we assessed Cre-mediated recomb nation efficiency, specificity, tamoxifen-dependent controllability, and functionality in both sexes. Both constitutive (Myh11-CreNLSP2A) and inducible (Myh11-CreERT2-P2A) Cre mice ...

2023年7月6日 · UST00000031320.7) is selected Ø Pf4-201 gene has 3 exons, with the ATG start codon at exon. and TAG stop codon at exon3. Ø We make Pf4-P2A-iCre knocki. mice via CRISPR/Cas9 system. Cas9 mRNA, sgRNA and donor will be co-injected into zygotes. sgRNA direct Cas9 endonuclease cleavage near stop codon(TAG) of Pf4 gene, and crea.

全身性基因敲除KO （Conventional Knockout）小鼠，也叫完全基因敲除小鼠，通过删除靶基因部分或全部参与翻译表达的功能域，KO小鼠全身上下甚至每根毛发里目的基因都无法完整正常表达，少了这个目的基因的正常表达，就能研究相应的生理和病理会出现的改变。 首先，是ES细胞打靶KO模型，它的策略通常是长这样子的： 这种模型（KOn）一般有一个（并不）可爱的 neo基因 （neomycin）作为标记，影响未知存在影响实验结果的风险（建议消灭），如果要快速得 …

Myh11-CreER T2-P2A KI mice were crossbred with mT/mG or LacZ reporter mice. Mice were intraperitoneally administered 50mg/kg/day tamoxifen/corn oil for five consecutive days, followed by a...

(A) Myh11-CreNLS P2A KI and SMMHCCreER T2 -Tg mice were crossbred with mT/mG reporter mice. Mice were intraperitoneally administered 50mg/kg/day tamoxifen/corn oil for five consecutive...