Our bioinformatic analysis indicated that DNMT isoforms interact with several transcription factors including p53, SP1, SP3, E2, p300, E47. Transcription factors such as PBX1 and PAX6 were found to interact with only DNMT1, NRSF1, STAT1 with DNMT3A, XBP1 and HFH1 with DNMT3B and SOX5, GFI1, MAX, PPARA with only DNMT3L.

Here we report that the histone deacetylase SIRT1 regulates the activities of DNMT1, a key enzyme responsible for DNA methylation. In mass spectrometry analysis, 12 new acetylated lysine sites were identified in DNMT1. SIRT1 physically associates with DNMT1 and can deacetylate acetylated DNMT1 in vitro and in vivo.

2015年12月15日 · Here we unveil that exposure to LIF initiates an epigenetic switch leading to the constitutive activation of JAK1/STAT3 signalling, which results in sustained proinvasive activity of CAF....

2003年11月24日 · Our results indicate that expression of the Dnmt 1 gene is controled by Sp1 and Sp3 and that p300 is involved in the activation by Sp3. histone acetyltransferase. Transcription is regulated by the combinational actions of proteins that bind to distinct promoter and enhancer elements.

该系统允许多达四个TALE-VP64基因快速装配成单个T-DNA载体，以同时激活植物中多达四个基因。 通过在拟南芥中靶向PAP1（图4），作者证实mTALE-act可以有效地激活内源基因表达。 这可能和TALE蛋白本身就是天然的基因转录激活子有关 (Lowder et al., 2018)。 在拟南芥中使用mTALE-Act观察到的强烈的转录激活与之前在人细胞和果蝇中使用TALE-VP64的研究一致。 因此，mTALE-Act系统是一个强大的转录激活系统，可同时上调植物中的多个基因。 图4 基 …

2015年12月15日 · We show that LIF induces constitutive activation of the JAK1/STAT3 signalling pathway by post-translational regulation of STAT3 acetylation by p300, in fibroblasts. Acetylated STAT3 leads to an epigenetic-dependent loss of expression of the SHP-1 tyrosine phosphatase, which is a negative regulator of the JAK/STAT pathway.

2019年4月16日 · We show here that treating RANKL-induced osteoclast progenitor (OCP) cells with the DNMT inhibitor 5-Aza-2′-deoxycytidine (5-Aza-CdR) induces CpG island hypomethylation and facilitates MMP-9 transcription. This increase in MMP-9 expression results in a significant enhancement of H3NT proteolysis and OCP cell differentiation.

The expression of Dnmt1 gene is observed at early S phase. Sp1 is recruited mainly at the G1 phase and Sp3 is recruited at the early S phase. p300 is also obviously recruited at the second S phase. These data indicated that the regulators of Dnmt1 gene were controlled in cell-cycle dependent manner.

2017年3月5日 · Major finding: Oxidative stress targets TET2 and DNMT1 to chromatin to increase both DNA methylation and 5hmC. Mechanism: p300-mediated acetylation of TET2 at K110 enhances TET2 activity, stability, and binding to DNMT1. Impact: HDAC inhibitors may enhance TET2 activity to suppress abnormal DNA methylation in patients with cancer.

Increased CtBP1/2 acts as transcriptional corepressors that, along with histone acetyltransferase p300 and Runt-related transcription factor 2 (Runx2), suppress the expression levels of six genes involved in bone healing: BGLAP (bone gamma-carboxyglutamate protein), ALPL (alkaline phosphatase), SPP1 (secreted phosphoprotein 1), COL1A1 (collagen ...