2007年10月1日 · In this report, we demonstrate that p300 was able to induce cell cycle arrest, and this process was reversed by p16 INK4a silencing by RNA interference in HeLa cells. We also show that p300...

present evidence that p300 participated in Sp1-mediated p16 transcription, and discuss the possible mechanism of the functional interaction between Sp1 and p300 in p16 transcriptional...

2011年9月13日 · In this study, inhibition or loss of p300, a histone acetyltransferase, is shown to result in senescence that occurs independently of p16 and is associated with histone hypoacetylation and...

2008年3月20日 · As a cyclin-dependent kinase inhibitor, p16(INK4a) plays a key role in cell cycle progression and cellular differentiation, and its expression is frequently altered in human cancers through epigenetically mediated transcriptional silencing. In this report, we demonstrate that p300 was able to induce …

2021年10月22日 · Acetyltransferase p300 plays significant roles in numerous cellular processes including proliferation, migration, differentiation, senescence, and apoptosis through chromatin remodeling in the regulatory regions of genes as an epigenetic regulator and/or an interacting coactivator with specific transcription factors of genes involved in those ...

In general, our study found increased expressions of p16 and p300 in peripheral blood EPCs of COPD patients, and CSE would lead to the increase of p16. In addition, p300/Sp1 enhanced the histone H4 acetylation level in the p16 promoter region to mediate the senescence of EPCs.

CSE介导的p16高表达促进细胞衰老。 COPD患者外周血EPCs中p300表达升高。此外，p300/Sp1增强了p16启动子区域的组蛋白H4乙酰化水平，从而介导EPC的衰老。敲除 p300/Sp1 可以挽救 CSE 介导的细胞衰老。结论。 p300/Sp1 增强 p16 启动子区组蛋白 H4 乙酰化水平，介 …

2008年3月20日 · In this report, we demonstrate that p300 was able to induce cell cycle arrest, and this process was reversed by p16INK4a silencing by RNA interference in HeLa cells. We also show that p300 was involved in activation of p16INK4a expression in 293T cells.

2021年8月19日 · The CSE-mediated high expression of p16 promoted cell senescence. The expression of p300 was increased in peripheral blood EPCs of COPD patients. Moreover, p300/Sp1 enhanced the histone H4 acetylation level in the promoter region of p16, thereby mediating the senescence of EPCs. And knockdown of …

针对Sp1和p300设计的siRNA能够通过部分地抑制Sp1和p300的表达而抑制p16 INK4a 启动子的活性。免疫共沉淀（CoIP）和哺乳动物双杂交实验表明,Sp1和p300通过Sp1的N末端和p300的Q区的相互作用而存在于同一复合物中。