2024年7月1日 · We showed that small molecule p300 inhibition can suppress fusion proteins-induced oncogenic transformation both in vitro and in vivo. Overall, our study reveals a molecular basis for VGLL involvement in cancer and provides a framework for targeting tumors carrying VGLL, TEAD, or NCOA translocations.

YAP可以强烈诱导乙酰转移酶P300对E3泛素连接酶SKP2的乙酰化，后者的乙酰化形式在细胞质中定位，造成其核内底物周期蛋白依赖性激酶抑制蛋白P27在核内大量累积，导致细胞有丝分裂阻滞并成为多倍体。

2024年3月27日 · 具体来说，乙酰转移酶p300被认为是一种乳酸转移酶，因此介导组蛋白的乳酸化，但缺乏使用纯化的p300蛋白和底物的直接体外证据。 更重要的是，在提出的催化系统中，p300需要使用乳酸辅酶A (lactyl-CoA)作为乳酸供体，但在哺乳动物细胞中负责产生乳酸辅酶A的酶仍然不明确，并且肿瘤细胞中乳酸辅酶A的水平几乎无法检测到。 事实上，细胞内乳酸辅酶A的浓度至少比哺乳动物细胞中的乙酰辅酶A低1000倍，这可能会大大限制p300在体内的乙酰转移 …

2023年2月1日 · Most recently, we demonstrated that both mouse and human PXR activation could promote liver enlargement and regeneration via yes-associated protein (YAP) signalling. PXR activation increased not only YAP expression but also the nuclear translocation of YAP, accompanied by the upregulation of YAP downstream genes [6].

2024年1月15日 · In this study, we found that EP300, one histone acetyltransferase, interacted with YAP and was recruited into the phase separated condensates of YAP. Transcriptomic analysis revealed substantial alterations in gene expression upon EP300 depletion, with downregulated genes associated with cancer progression and Hippo-YAP pathway.

2024年6月19日 · 报告简介： The Hippo pathway effector YAP plays an essential role in cardiomyocyte proliferation and heart regeneration. How YAP responds to physiological conditions such as myocardial infarction...

通过Western blot、SA-β-Gal染色、Masson染色和免疫荧光检测Piezo1、YAP和p300以及衰老和纤维化相关蛋白的表达，采用心脏超声观察小鼠的心功能变化，经颈静脉在体电刺激检测各项电生理指标和房颤可诱发性。

2024年5月1日 · We showed that small molecule p300 inhibition can suppress fusion proteins-induced oncogenic transformation both in vitro and in vivo. Overall, our study reveals a molecular basis for VGLL involvement in cancer and provides a framework for targeting tumors carrying VGLL, TEAD, or NCOA translocations.

2024年5月1日 · Furthermore, we identify that VGLL2 and TEAD1 fusions engage specific epigenetic regulation by recruiting histone acetyltransferase p300 to control TEAD-mediated transcriptional and epigenetic landscapes. We showed that small molecule p300 inhibition can suppress fusion proteins-induced oncogenic transformation both in vitro and in vivo .

多泛素化的DDX17与p300-YAP的结合增加，导致与干性相关基因近端的组蛋白3赖氨酸56（H3K56）乙酰化，以及它们随后的转录激活。