2005年9月1日 · In this review, we present and discuss recent knowledge about p40 phox, the last NADPH oxidase component to be identified. Furthermore, its interaction with cellular pathways outside of the NADPH oxidase is discussed.

PX 结构域见于超过 250 个种蛋白，包括 NADPH 氧化酶复合体的 p40 phox 和 p47 phox 组分、分选连接蛋白、磷脂酶 D1 和 2 以及激酶 PI3K 和 CISK。 PX 结构域包括一个氨基末端三链 β 片层以及一个由四个 α 螺旋构成的 α 螺旋状亚结构域。 PX 结构域优先结合 PtdIns (3)P，但有些被报道会结合其它磷酸肌醇。 含 PX 结构域的蛋白与各种功能有关，包括蛋白质分选、囊泡运输和磷脂代谢。 现已确定 PX 结构域与磷酸肌醇相互作用的结构基础在于包含 PI (3)P 的复合体中 p40 …

2001年6月12日 · PX domains, named for their presence in the p40phox and p47phox subunits of the NADPH oxidase 1, are ∼ 140-amino-acid-long modules found in more than 150 eukaryotic proteins, including 45...

In this review, we present and discuss recent knowledge about p40phox, the last NADPH oxidase component to be identified. Furthermore, its interaction with cellular pathways outside of the NADPH oxidase is discussed.

2023年6月23日 · P40 phox has a special function in this process: it directs the NOX2 complex to the membranes of phagosomes, the vacuoles that contain ingested microorganisms, thus ensuring superoxide formation in close vicinity to the pathogenic targets.

We show here that a novel component, p40phox, forms an activation complex with p47phox and p67phox with which it translocates to the membrane to associate with the flavocytochrome b. cDNA cloning and amino acid analysis revealed that p40phox has an src homology 3 (SH3) domain and a large region of sequence similarity with the N-terminus of p47phox.

Biallelic loss-of-function (LOF) mutations of the NCF4 gene, encoding the p40 phox subunit of the phagocyte NADPH oxidase, have been described in only 1 patient. We report on 24 p40 phox -deficient patients from 12 additional families in 8 countries.

Here we have explored the interaction between the actin cytoskeleton and p40 phox, one of the cytosolic components of NADPH oxidase. Full length p40 phox expressed in COS cells co-localised with F-actin in a peripheral lamellar compartment.

2005年9月1日 · In this review, we present and discuss recent knowledge about p40phox, the last NADPH oxidase component to be identified. Furthermore, its interaction with cellular pathways outside of the NADPH...

Here we show that the PtdIns (3)P-binding activity of p40 phox is normally inhibited by the PB1 domain both in vivo and in vitro. The crystal structure of the full-length p40 phox reveals that the inhibition is mediated via intramolecular interaction between the PB1 and PX domains.