Phospho-Akt (Ser473) (736E11) Rabbit mAb detects Akt1 only when phosphorylated at serine 473, and Akt2 and Akt3 only when phosphorylated at equivalent sites. This antibody is not approved for traditional western blot analysis.

Phospho-Akt (Ser473) Antibody (IHC Specific) detects Akt1 only when phosphorylated at serine 473, and Akt2 and Akt3 only when phosphorylated at equivalent sites. This antibody was developed for and is recommended for immunohistochemical application only.

Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery.

Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or p70 S6 kinase.

Each control slide contains formalin fixed, paraffin-embedded LNCaP cells, both untreated and treated with PI3-Kinase inhibitor LY294002, that serve as a control for Phospho-Akt (Ser473) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the LY942002 treatment.

Invitrogen Anti-Phospho-AKT1 (Ser473) Monoclonal (14-6), Catalog # 44-621G. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (0.5 mg/mL).

Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Phosphorylated at Thr-308 and Ser-473 by IKBKE and TBK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A).

免疫组织化方法验证Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb抗体，使用的切片为SignalSlide® Phospho-Akt (Ser473) IHC Control#8101(石蜡包埋的LNCaP细胞），左图为未处理的LNCaP细胞，右图为LY294002处理的LNCaP细胞。

AKT (also known as protein kinase B alpha) is a 60 kD serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation.

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.