2017年12月1日 · Stability of p53 is regulated by the ubiquitin-proteasome pathway (UPP). Furthermore, it is readjusted by deubiquitination via deubiquitinating enzymes (DUBs) that can eliminate ubiquitin from p53. Diverse DUBs directly or indirectly affect the ubiquitination of p53 and, consequently, regulate various cellular processes associated with p53.

USP10 is identified as a cytoplasmic DUB that regulates p53 levels and localization by reversing Mdm2-induced p53 nuclear export and degradation. It regulates the stability of p53 without interacting with MDM2, unlike USP2 and USP7, and has been shown to stabilize the wild type as well as the mutated form of p53, playing a dual role in ...

该途径也是一种动态的蛋白质双向修饰调控系统，在体内由 泛素连接酶系统 （E1-E2-E3）对底物进行泛素化修饰，去泛素化酶（DUB）家族负责通过水解泛素羧基末端的酯键、肽键或异肽键，将泛素分子特异性的从链接有泛素的蛋白质或者前体蛋白水解下来，起到去 ...

In this study, we have identified OTUD5 as a DUB that interacts with and deubiquitinates p53. OTUD5 forms a direct complex with p53 and controls level of ubiquitination. The function of OTUD5 is required to allow the rapid activation of p53-dependent transcription and a p53-dependent apoptosis in response to DNA damage stress.

2014年5月26日 · A number of deubiquitinating enzymes (DUBs) from the ubiquitin-specific protease family have been shown to regulate the p53-MDM2-MDMX networks. We recently reported that Otub1, a DUB from the OTU-domain containing protease family, is …

2024年6月30日 · Here, using small-molecule microarray-based technology and unbiased screening, we identified three potential molecular glues that may tether P53 to the DUB, USP7, and elevate the P53 level. Among the molecular glues, bromocriptine (BC) is an FDA-approved drug with the most robust effects.

2013年10月15日 · Principal findings: In this study, we have identified OTUD5 as a DUB that interacts with and deubiquitinates p53. OTUD5 forms a direct complex with p53 and controls level of ubiquitination. The function of OTUD5 is required to allow the rapid activation of p53-dependent transcription and a p53-dependent apoptosis in response to DNA damage stress.

2025年3月11日 · In this study, we demonstrated the involvement of DUBs—USP5 and USP9X in—enhancing mutp53 stability while revealing the effects of DUB inhibitor WP1130 in selectively destabilizing different p53 mutants in cancer cells of various origins.

Abstract as emerged as an important mecha-nism of p53 regulation. A number of deubiquitinating enzymes (DUBs) from the ubiquitin-specific protease famil have been shown to regulate the p53-MDM2-MDMX networks. We recently reported that Otub1, a DUB from the OTU-dom

2025年3月11日 · In gastric cancer cells harbouring p53 mutations, ovarian tumour domain-containing 5 (OTUD5) transcription is upregulated due to the inactivation of p53, allowing OTUD5 to stabilise glutathione perox...