Through physical interaction with p53, p300/CBP can both positively and negatively regulate p53 transactivation, as well as p53 protein turnover depending on cellular context and environmental stimuli, such as DNA damage.

1997年6月27日 · In this study, we show that p53 and p300 form a specific protein complex. We demonstrate that p53-mediated inhibition of AP1-regulated enhancers and transcriptional activation of promoters containing a p53 consensus site are both p300 dependent.

1997年6月19日 · Here we report that p300/CBP binds to p53 in the absence of viral oncoproteins, and that p300 and p53 colocalize within the nucleus and coexist in a stable DNA-binding complex. Consistent...

2019年3月1日 · The acetyltransferase p300 was required for p53-dependent activation of CDKN1A in vitro and in cells 79,171 and led to an increase in the gene-activating histone H4...

We show here that p300 stabilizes both p53 and its negative regulator MDM2, thereby enhancing the p53/MDM2 negative regulatory loop. Binding of p300 is associated with the retention of p53 in the nucleus, which results in the accumulation of p53 in an acetylase-independent manner.

2019年11月28日 · It has been established that phosphorylated p53 is released from Mdm2 and binds to p300, orchestrating the transcriptional program. We investigate whether molecules such as ALRN_6924 would bind to p300 and, to do so, we used molecular simulations to explore the binding of ATSP_7041, which is an analogue of ALRN_6924.

2000年11月7日 · p300 acetylates and activates the tumor suppressor p53 after DNA damage. Here, we show that MDM2, a negative-feedback regulator of p53, inhibited p300-mediated p53 acetylation by complexing with th...

Adenovirus E1A stabilization and repression of p53 activity via p300 binding prompted us to investigate the role of p300 in p53 turnover. The p300 N-terminal region was found to reflect certain structural and functional properties of human papilloma virus E6.

2001年3月15日 · Functionally, inhibition of deacetylation promotes p53 stability, suggesting that acetylation plays a positive role in the accumulation of p53 protein in stress response. Our results provide evidence that p300/CBP-mediated acetylation may be a universal and critical modification for p53 function.

2001年3月3日 · Here we demonstrate that, unlike phosphorylation, p53 invariably undergoes acetylation in cells exposed to a variety of stress-inducing agents including hypoxia, anti-metabolites, nuclear export inhibitor and actinomycin D treatment. In vivo, p53 acetylation is mediated by the p300 and CBP acetyltransferases.