Nuclear NF-κB expression was elevated 30-min following LPS treatment, maximal by 45-min, and returned to basal levels by 24-h. Binding of nuclear NF-κB to consensus oligonucleotide …

NF-κB p65 是NF-κB家族成员之一，NF-κB是一种多效性转录因子，几乎存在于所有细胞类型中，并参与许多生物学过程，例如炎症、免疫、分化、细胞生长、肿瘤发生和凋亡。 NF-κB受翻 …

2021年3月1日 · SNX25 knockdown decreased cytoplasmic p65 after 30 min LPS stimulation (SNX25 siRNA, 0.74 ± 0.19-fold control P = 0.012). In addition, SNX25 knockdown increased …

Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein …

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was …

LPS作用30min后，Western blotting 检测不同实验组细胞核、细胞质内NF-κB p65蛋白水平，LPS作用9h后，实时荧光定量PCR法检测NF-κB p65、COX-2 mRNA水平。 结果 LPS作 …

2025年3月4日 · A previous study showed that p65 phosphorylation at Ser 276 facilitated its interaction with p300/CREB (adenosine 3′,5′-monophosphate response ... for 30 min, and the …

1 天前 · NF-κB p65 remains inactive in the cytoplasm until activated, upon which it is rapidly imported into the nucleus by importin family proteins. ... The mixture was vortexed and …

之前做0，30，60min也没跑出来，可能时间长了磷酸化已经结束了，后来一直跑的0，15，30min，多试几个时间点

2024年10月30日 · The nuclear translocation of p65 was detected using immunofluorescence staining. After washing with PBS, Raw264.7 cells in each group was successively fixed with …