RELA, or v-rel avian reticuloendotheliosis viral oncogene homolog A, is also known as p65 or NFKB3. [7] It is located on chromosome 11 q13, and its nucleotide sequence is 1473 nucleotide long. [8] RELA protein has four isoforms, the longest and the …

2024年12月25日 · 本文将从p65蛋白的结构特性、生理功能、调控机制及其在疾病中的作用等方面，为读者呈现这一生物信号传导领域的“明星分子”。 p65蛋白，也被称为RelA，是NF-κB家族中的一个关键转录因子。 NF-κB家族包括P50、P52、RelA（p65）、c-Rel和RelB五个成员，它们均含有Rel样结构域，是参与多种生物学过程的多效性转录因子。 p65蛋白因其在SDS-PAGE检测中显示的相对分子质量为65×10³而得名，其实际分子量在不同条件下可能有所变化，这与其翻译后 …

Hence, the NF-κB p65 signaling pathway has been a pivotal point for intense drug discovery and development. This review begins with an overview of p65-mediated signaling followed by discussion of strategies that directly target NF-κB p65 in the context of chronic inflammation. Keywords: NF-κB, inflammation, therapy.

2024年9月6日 · Our work identifies p65 as a previously unrecognized contributor to cell cycle regulation, revealing why and when pro-inflammatory signaling is required during HSPC development. It highlights...

2 天之前 · Similarly, SFRP2, αSMA, and phosphorylated p65 levels were elevated following recombinant PAI-1 exposure in both the human hepatic stellate cell line LX2 (i.e., hepatic pericytes; Figures S6Q–S6T) and a murine pericyte model (Figure S6U), whereas silencing LRP1 or p65 in LX2 cells reversed these changes (Figures S6R–S6T).

1 天前 · Immunofluorescence analysis of NF-κB p65 expression in BMDMs from WT and Pad2 −/− mice treated with PA-derived LPS (250 ng mL −1, 1 h) compared to control (n = 5), with cells stained for NF-κB p65 (in red) and nuclei counterstained with DAPI (in blue) under a confocal microscope. Relative protein expression levels are shown in the right ...

2024年9月6日 · Our work identifies p65 as a previously unrecognized contributor to cell cycle regulation, revealing why and when pro-inflammatory signaling is required during HSPC development. It highlights the importance of considering and leveraging cell signaling as a temporally dynamic entity.

2024年7月9日 · Understanding p65 post-translational modifications not only elucidates NF-κB pathway regulation but also facilitates the identification of therapeutic targets and diagnostic markers for associated clinical conditions.

2025年3月4日 · The cells were lysed with lysis buffer (10% NP-40, 50 mM Hepes, 150 mM sodium chloride, 1 mM EDTA, and protease and phosphatase inhibitors) and centrifuged at 13,000 rpm and 4°C for 30 min. Next, 500 μg of cell lysates was incubated with 5 μg of anti-p65 or anti-GCN5 antibodies at 4°C overnight.

Our findings show that RelA/p65−/− murine fibroblasts immortalize at considerably faster rates than RelA/p65+/+ cells. The ability of RelA/p65−/− fibroblasts to escape senescence earlier is due to their genomic instability, characterized by high frequencies of DNA mutations, gene deletions and gross chromosomal translocations.