2018年10月28日 · Using the RDG platform, we developed a hybrid, multimodal nanotherapeutic system that incorporates the anti-NF-κB agent, p65-shRNA, for the effective treatment of metastatic breast cancer. As shown in Scheme 1, this multimodal nanocomplex, RDG/shRNA, was prepared by complexing cationic DSPEI with negatively charged p65-shRNA.

A knock down of endogenous Grx-1 by siRNA or pretreatment with an inhibitor of Grx-1 activity, CdCl(2), abolishes p65-SSG formation. In addition, Grx-1 siRNA blocks the inhibition of p65 nuclear translocation and ICAM-1 expression, suggesting that this enzyme is involved in the cinnamaldehyde-mediated NF-kappaB inhibition.

2017年10月9日 · Here we demonstrated that p65 can bind to HSC70 with four consensus recognition motif in its RHD domain and be constitutively transported to the lysosome membrane to bind with lysosome-associated...

2018年10月28日 · This multimodal therapeutic system was prepared by complexing RDG nanovectors with p65-shRNA, an anti-NF-κB agent, via the electrostatic interactions between negatively charged shRNA and the cationic DSPEI displayed on the surface of the nanovectors.

Because 15-deoxy-Δ12,14-prostaglandin J 2 (15 d-PGJ 2) is an electrophilic prostaglandin that can increase glutathione (GSH) levels and augment reactive oxygen species (ROS) production, we hypothesized that it induces NF-κB-p65 glutathionylation and would …

2024年3月13日 · Using a microglial culture supernatant (MCS) transfer model, we found that inhibiting LRRK2 or downregulating ferroptosis in BV2 cells prevented SH-SY5Y cell apoptosis. Additionally, we observed abundant expression of LRRK2 and P-P65 in the midbrain, which was elevated in the MPTP-induced PD model, along with microglia activation.

Download scientific diagram | RNAi modulation of Nrf2, Keap1 and p65 influences the level of GSH. Cells were transfected for 48h with RNAi and treated for 6h with DNCB. Keap1 depletion...

通过从NIR激光照射和高细胞内GSH浓度双重刺激，可实现复合物中shRNA的胞质释放，从而有效地抑制转移性4T1乳腺癌细胞的基因沉默，从而抑制细胞增殖和侵袭。 更重要的是，由于EPR效应和RGD介导的内吞作用，纳米复合物会发生明显的肿瘤内蓄积。 结合局部NIR激光照射，该杂合复合物可以有效抑制原发性乳腺肿瘤的生长，几乎完全抑制远处转移，从而显着提高RDG / shRNA复合物的治疗效果。 因此，这种具有肿瘤靶向能力和胞质shRNA释放的NIR-light和GSH反应复 …

2018年10月28日 · This multimodal therapeutic system was prepared by complexing RDGs with shRNA through electrostatic interactions between cationic DSPEI and negatively charged p65-shRNA. The therapeutic nanosystem exhibited controlled shRNA release triggered by intracellular high GSH and NIR laser irradiation, resulting in effective gene silencing

We explored the underlying mechanism of Glrx in the pathogenesis of LAM. Our data suggested that Rapamycin‐independently hyperactivation of Glrx in LAM attenuated intracellular oxidative stress and Bim‐mediated apoptosis, which is related to decreased GSH adducts on p65 nuclear factor‐kappa B (NF‐κB).