Microglial HMGB1/STAT3/p65 axis within the mPFC is a novel therapeutic target in the treatment of MDD associated with stress. Neuroinflammation and autophagy are implicated in stress-related major depressive disorder (MDD), but the underlying molecular …

2025年2月6日 · Immunofluorescence staining tested co-localization between HO-1 or NF-κB p65 and IBA1. The expressions of gene and proteins were detected by RT-qPCR and Western blot, respectively. Flow cytometry determined microglial polarization phenotype and neuron apoptosis.

These results suggest that CA exerts a neuroprotective effect in acute PD model mice by suppressing glial cell activation, modulating the expression of apoptotic genes, and alleviating neuroinflammation and apoptosis induced by MPTP. Keywords: cinnamaldehyde, GDNF, GFAP, IBA1, NF- κ B p65, Parkinson's disease. 1. Introduction.

2024年5月8日 · Luciferase reporter assay showed that inhibiting p65 using its specific inhibitor BAY 11–7082 or silencing HIF1α using siRNAs reduced the transcriptional activity of SLC15A3 in LPS/OGD-induced BV2 cells. Results in NIH 3T3 cells also confirmed that p65 and HIF1α directly bound to the SLC15A3 promoter to activate SLC15A3 transcription.

2019年4月8日 · We found that LPS treatment leads to sickness behavior and cognitive impairment in mice as shown in the Morris water maze and passive avoidance test, and these effects were accompanied by microglia...

2022年10月1日 · Recently, the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway has emerged as a key mediator of inflammation and shows potential in modulating microglial polarization. In this study, we evaluated whether cGAS-STING is a potential therapeutic target.

2022年8月2日 · The study showed that both siRNA silencing of Dyrk1A and harmine treatment inhibited nuclear translocation of p65 in LPS-mediated BV2 microglia cells and ICR mice, respectively. In addition, TLR4, an upstream receptor protein of the NF-κB p65 pathway, plays a vital role in the exploration of anti-inflammatory mechanisms .

Nuclear factor κB p65 was upregulated in M1 microglia and pro-inflammatory astrocytes, while Stat6 was upregulated in M2 microglia and anti-inflammatory astrocytes. These results provide direct evidence that microglial polarization governs communication between microglia and astrocytes, and that AD debris alters this crosstalk.

2025年3月3日 · 与模型组比较,洗心汤血清组细胞存活率明显升高,细胞上清液s100β、nf-κb p65含量减少,iba1、dectin-1阳性表达明显减少,tnf-α mrna表达减少、tgf-β mrna表达升高,差异有统计学意义(均p0.01)。结论:洗心汤含药血清通过下调iba1、tnf-α、s100β、nf-κb p65、dectin-1的表达,增 …

Activated IKKβ phosphorylates the IκB inhibitory protein IκBα, targeting it for ubiquitination and proteasomal degradation, and subsequent release of NF-κB (p65/p50) to the nucleus where it binds to its cognate DNA sequences to induce gene expression (Ghosh and Karin, 2002).