2023年3月30日 · IκBα磷酸化 激活与降解， p65/p50 入核，促进炎症因子产生（ IL1β 、 TNF-α 、 IL-6 等） 非经典NF-kB通路：慢而持久，主要由TNF受体家族激活。 RelB/p52 入核。 家族成员： ps：p105和p100分别是p50和p52的前体蛋白。

2024年12月30日 · TNF-alpha是一种重要的炎症介质，能够诱导RELA/NF-KB p65的激活和核转运，进而促进炎症反应。 NLRP3通过抑制TNF-alpha的活性，可以间接地影响NF-KB信号通路的激活状态。

NF-κB p65的翻译后修饰可能需要条件诱导，例如Calyculin A和TNF-a刺激细胞后，可检测到536位丝氨酸被磷酸化修 饰的NF-κB p65蛋白。 由于NF-κB p65存在这些翻译后修饰，因此在WB实验时，这些翻译后修饰可能会影响NF-κB p65蛋白大小和条带数目。

Our data reveal the temporal kinetics of pro- and anti-inflammatory signaling events that may be important therapeutic targets for inflammatory diseases. Keywords: Inflammation; TLR4; interleukin-10; lipopolysaccharide; nf-kappa B; tumor necrosis factor. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.

NF-κB p65的翻译后修饰可能需要条件诱导，例如Calyculin A和TNF-a刺激细胞后，可检测到536位丝氨酸被磷酸化修饰的NF-κB p65蛋白。 使用新鲜制备的样本，防止冻存样本导致磷酸化修饰减弱。

Phosphorylation of p65 at T254 by an unknown kinase is induced by TNFα, and results in a phosphorylated threonine-proline motif that is recognised by the peptidyl-prolyl isomerase Pin1. Pin1-mediated prolyl isomerisation of p65 leads to reduced IκBα binding, p65 nuclear accumulation, increased p65 stability and enhanced transcription [77,78 ...

2012年2月1日 · Here we report that TNFα acts through NFκB signaling and requires a conserved promoter region for the down-regulation of Cldn5 expression. Overexpression of the NFκB subunit p65 (RelA) alone repressed Cldn5 promoter activity in mouse brain endothelial cells.

NLRP3抑制TNF-alpha，TNF-alpha诱导RELA/NF-KB p65的激活和核转运。 NLRP3激活caspase-1，响应细菌或病毒感染等多种触发因素，导致IL1B和IL18的加工和释放。 炎症小体通路（图2）。

2013年8月1日 · Our data showed that knockdown of KPNB1, XPO7 and IPO8 reduced the amount of nuclear p65 following tumor necrosis factor-α (TNF-α) stimulation, resulting in lower NF-κB activity. KPNB1 was the major importin β receptor for p65 import, and this import was dependent on the NLS of p65.

2025年3月4日 · A previous study showed that p65 phosphorylation at Ser 276 facilitated its interaction with p300/CREB (adenosine 3′,5′-monophosphate response element–binding protein)–binding protein ... Cox-2, Tnf-α, and Il-6 mRNA levels were significantly higher 6 hours after LPS treatment than PBS treatment (P < 0.0001).