2023年6月23日 · p67 phox-mediated activation of the phagocyte oxidase Nox2. In simulated phagocytes, p67 phox translocates to the plasma membrane via p47 phox, whose bis-SH3 domain and PX domain bind to the transmembrane protein p22 phox and phosphoinositides (PIPs), respectively.

2010年1月1日 · In this study, we report the SAXS analysis of p67 phox. Our data reveals that p67 phox behaves as a multidomain protein with semi-flexible linkers. On the one hand, it appears to be a very elongated molecule with its various domains organized as beads on a string.

他们利用冷冻电镜技术解析了激活和静息状态下NOX2的结构，并在原子水平上揭示了胞浆组分p67、 Rac1 和p47与NOX2的结合方式及引发的构象变化。这一发现为深入探究NOX2的激活机制奠定了坚实的结构基础。

2022年11月22日，北京大学未来技术学院分子医学研究所，北大-清华生命科学联合中心陈雷研究员研究组在 ELife 杂志上发表了题为“ Structure of human phagocyte NADPH oxidase in the resting state ”的研究论文，该研究通过单颗粒冷冻电镜技术解析了人源吞噬细胞NADPH氧化酶复合体在静息状态下的高分辨结构。...

2011年11月1日 · In this study, we show that p67phox is constitutively phosphorylated in resting human neutrophils and that neutrophil stimulation with PMA further enhanced this phosphorylation. Inhibition of the constitutively active serine/threonine phosphatases type 1 and type 2A (PP1/2A) by calyculin A resulted in the enhancement of p67phox phosphorylation.

To study this, we measured binding of p67 phox to a library of Nox2 peptides and binding of NusA-Nox2 fusion proteins to p67 phox. We found, serendipitously, that deletion of residues 259-279 in p67 phox (p67 phox Δ(259-279)), endowed it with the ability to bind selectively to Nox2 peptide 369-383 (peptide 28).

We recently identified an auto-inhibitory intramolecular bond in p67 phox, one extremity of which was located within the 259-279 sequence, and we hypothesized that inhibition by exogenous peptides might mimic intrinsic auto-inhibition.

p67phox Antibody detects endogenous levels of total p67phox protein. Human, Mouse, Rat. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala25 of human p67phox. Antibodies are purified by protein A and peptide affinity chromatography.

The NADPH oxidase complex is involved in the destruction of phagocytosed pathogens through the production of reactive oxygen species. This activatable complex consists of a membranous heterodimeric flavocytochrome b, a small G-protein Rac1/Rac2 and cytosolic factors, p47(phox), p67(phox) and p40(pho …

2021年3月22日 · p67-phox Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects p67-phox of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p67-phox Antibody (D-6) is ...