This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter).

APC-Cy7是流式细胞术中常用的荧光染料组合，其最大吸收峰位于651 nm，发射峰约780 nm。AAT Bioquest的还提供了ReadiUse™预活化APC-Cy7串联染料，可高效偶联抗体、链霉亲和素及其他二级试剂等蛋白质。 其偶联效率显著高于SMCC化学偶联法。

APC-Cy7 (APC-Cyanine 7) has an excitation peak at 650 nm and an emission peak at 785 nm, and is spectrally similar to APC-CF®750 (Biotium), APC-H7 (BD Biosciences), APC-eFluor™ 780 (Thermo Fisher Scientific), APC-Vio®770 (Miltenyi Biotec) and APC-Fire™ 750 (BioLegend). This dye is a member of the Cyanine® dye family, a trademark of GE heathcare.

apc-cy7通常用于标记dna、rna和蛋白质等生物分子，以便在显微镜下观察或进行流式细胞分析。 APC-Cy7可以与抗体结合，用于标记细胞表面的抗原，也可以用于检测抗原-抗体反应。

APC-Cy7 复合荧光素是将交联别藻蓝蛋白Cl-APC 和有机荧光染料Cy7 通过化学偶联 制成，具有荧光强度高，Stokes 位移大的特点。 同时，利用复合荧光素具有APC 荧光强度

2023年11月23日 · 别藻蓝蛋白（apc）cy7，别藻蓝蛋白（apc）是从螺旋藻（一种蓝绿藻）中分离的藻胆蛋白。与其他藻胆蛋白一样，apc是荧光的，具有极高的吸收率和高量子效率。

This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter).

This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter).

(This mouse IgG1 kappa monoclonal antibody with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human …

2023年12月20日 · apc-cy7是一种荧光染料，常用于免疫细胞标记和追踪。 它是一种近红外荧光染料，具有较高的光稳定性和较低的背景干扰，因此被广泛应用于流式细胞术、免疫组织化学和荧光显微镜等实验中。