In the current study, we generated PB2-KO virus expressing PspA as a bivalent vaccine for influenza and pneumococcal pneumonia and examined whether intranasal immunization with this bivalent vaccine could induce influenza virus–specific and PspA-specific antibodies and afford protection from lethal infection with influenza virus or S. pneumoniae...

We recently developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that possesses a reporter gene (the green fluorescent protein gene) in the coding region of the PB2 segment. This virus replicated to high titers in PB2-expressing, but not unmodified, cells, suggesting its potential safety and feasibility as a vaccine.

2022年10月26日 · 甲型流感病毒(iav)聚合酶蛋白pb2已显示通过阻断干扰素(ifn)的诱导来部分抑制宿主免疫反应。 然而，调节IFNs下游信号通路的IAV PB2蛋白没有很好的表征。

2017年4月4日 · Virus-specific IgG and IgA antibodies were detected in mice immunized with the viruses, and the immunized mice showed milder clinical signs and/or lower virus replication levels in the respiratory tract upon virus challenge. Our results indicate that these PB2-KO viruses have potential as vaccine candidates. 1. Introduction.

In addition, these PB2-KO viruses efficiently elicited virus-specific IgA responses, which are associated with protection from virus challenge (Fig. 2) (24–27). However, the neutralizing antibody titers of the sera from mice immunized with PR8/PB2-VN1203HA or PR8/PB2-Ca04HA virus were below the detection limit ( Fig. 2 ).

We recently developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that possesses a reporter gene (the green fluorescent protein gene) in the coding region of the PB2 segment. This virus replicated to high titers in PB2-expressing, but not unmodified, cells, suggesting its potential safety and feasibility as a vaccine.

We recently developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that possesses a reporter gene (the green fluorescent protein gene) in the coding region of the PB2 segment. This virus replicated to high titers in PB2-expressing, but not unmodified, cells, suggesting its potential safety and feasibility as a vaccine.

文章对PB2蛋白的结构和功能特征及其在哺乳动物适应性机制方面的最新 研究进展进行归纳总结，为揭示流感病毒跨物种传播机制及相关防控手段的完善提供参考信息。 关键词: 流感病毒；PB2基因；跨物种传播；哺乳动物适应性；分子机制. 中图分类号: 郭雨欣，周栋梁，蒲 娟，张 蕊. PB2基因在流感病毒跨物种传播和哺乳动物 适应性中的作用及机制研究进展 [J]. 中国家禽, 2024, 46 (11): 121-131. 导出引用管理器 EndNote | Ris | BibTeX. 链接本文: …

2012年2月1日 · We recently developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that possesses a reporter gene (the green fluorescent protein gene) in the coding region of the PB2...

2022年10月21日 · Influenza A virus (IAV) polymerase protein PB2 has been shown to partially inhibit the host immune response by blocking the induction of interferons (IFNs). However, the IAV PB2 protein that...