2023年8月4日 · Potentiation of excitatory synaptic transmission in the parabrachial nucleus (PBN) to central amygdala (CeA) pathway has been reported in rodent models of persistent pain.

2022年2月15日 · The parabrachial nucleus (PBN) and thalamic paraventricular nucleus (PVT) are critical for modulating affective states in mice. However, the functional roles of PBN-PVT projections in modulating affective states remain elusive. Here, we show that PBN neurons send dense projection fibers to the PVT and form direct excitatory synapses with PVT ...

2020年11月25日 · Inhibition of glutamatergic LPBN neurons alleviates both basal nociception and neuropathic pain-like hypersensitivity. Repetitive pharmacogenetic activation of glutamatergic or GABAergic LPBN...

2023年2月21日 · AAV-DIO-ChR2:YFP was injected into the PBN of Oprm1 Cre/+ mice. MOR neurons were then activated with light via implanted optical fibers and changes in breathing activity were assessed under ...

2022年2月15日 · To examine the functional role of PBN-PVT projections in modulating affective behaviors, we injected AAV2/9-EF1α-DIO-ChR2-mCherry virus or AAV2/9-EF1α-DIO-mCherry virus bilaterally into the PBN of Vglut2-ires-Cre mice and implanted optic fibers above the PVT to selectively activate PBN-PVT projections (Figure 2A).

2023年8月2日 · We report a method to record from PBN neurons of behaving, head-restrained mice while applying reproducible noxious stimuli. We find that both spontaneous and evoked activity are higher in awake animals compared with urethane anesthetized mice.

2022年11月1日 · The Calca Cre mice were used to identify mRNAs that were selectively being translated in neurons that express the Calca gene by injecting a virus expressing a Cre-dependent hemagglutinin (HA)-tagged ribosomal protein 22 (AAV-DIO-Rpl22-HA) (Sanz et al., 2015) into the PBN of eight adult mice.

We report a method to record from PBN neurons of behaving, head-restrained mice, while applying reproducible noxious stimuli. We find that both spontaneous and evoked activity are higher in awake animals, compared to urethane anesthetized mice.

2024年1月26日 · Mice were injected with AAV-DIO-GCaMP6s virus into the LPBN and optical fibers were implanted over the LPBN to monitor the cellular activity of LPBN neurons in awake behaving mice (Fig. 1 (B,C)). We hypothesized that these …

Cartoon sagittal section of mouse brain showing the approximate locations of CGRP neurons in the parabrachial nucleus (PBN), their inputs from the spinal cord and the nucleus tractus solitarius (NTS) where CCK- and DBH-expressing neurons reside, and their axonal projections to the central nucleus of the amygdala (CeA) and the oval region of the ...