Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations.

In this work, we described a trainable segmentation algorithm for PCM images based on multi-scale local BIFs histograms. It performed well in foreground versus background segmentation tasks, approaching performance of state-of-the-art specialised algorithms.

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(A) (i) Cropped region of a mouse embryonic stem cell PCM image shortly after seeding. Insert shows a zoomed-in region with description of the key features of a typical PCM image including a halo artifact surrounding the cell and the lack of contrast between the background and the interior of the cell (ii) Cell contour detected (black line ...

一种基于双树复小波变换和经验梯度阈值的相衬显微细胞图像分割方法. Contribute to shd0126/Cell-segmentation-in-PCM-images-with-DTCWT-and-EGT development by creating an account on GitHub.

Phase contrast microscopy (PCM) images of Bessel beam induced high aspect ratio permanent structures in fused silica using single laser pulses of energy E =6 μJ and four...

Phase contrast microscopy (PCM) is widely used as the de facto light microscopy modality for the inspection of adherent cell cultures. PCM enables the observation of transparent cellular specimens by transforming phase shifts (induced by differences in refractive index between the sample and the surrounding medium) into changes in

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2017年9月3日 · Phase contrast microscopy (PCM) is routinely used for the inspection of adherent cell cultures in all fields of biology and biomedicine. Key decisions for experimental protocols are often taken by an operator based on typically qualitative observations.

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