2020年6月18日 · Multiplex qPCR requires careful design for optimal results. Choosing the correct qPCR dye combinations will enhance the precision and reduce the background for your assay. Use the IDT PrimeTime™ Multiplex Dye Selection Tool and the following recommendations to find the best dye combination for your assay.

In this study, we investigate 15 different intercalating DNA dyes for their inhibitory effects on PCR, effects on DNA melting temperature and possible preferential binding to GC-rich sequences.

Review these recommendations for selecting dyes for multiplex qPCR that minimize background and avoid overlap of uorescent signals. Included is a table of compatible dyes for multiplexing on common qPCR instruments and a list of suggested quenchers. The experimental design for multiplex qPCR is more complicated than for single reactions.

2015年4月7日 · Here we provide some advice on how to select appropriate dyes and quenchers for your probe-based qPCR assays. Selecting an appropriate reporter dye for qPCR analysis will depend on the type of instrument you are using and the …

2021年6月3日 · ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product. However, ROX fluorescence is affected by anything else that would alter overall fluorescence readings, such as:

自PCR技术成熟之后，DNA染色法被大量开发，我们想用最便宜、简单而且安全的方法进行最灵敏、有效的染色。我们下面就聊聊几种常见的染色剂。这些染色剂有的是荧光染料（Fluorescent dyes），有的是非荧光（Non-fluorescent dyes）。

2022年4月1日 · New dimeric dyes exhibit a strong fluorescence enhancement on binding to dsDNA along with good PCR instrument compatibility. Obtained dyes are non-toxic and demonstrate high thermal, photolytic and hydrolytic stability. Dyes 6a–6d are less inhibitory to qPCR than SYBR Green I at a broader range of dye concentrations (i.e. 0.75–1.75 μmol/L).

Recently issued a patent for its CAL Fluor™ dyes, we introduced this series of vibrant dyes for real-time qPCR as lower-cost, superior performing replacements for many commonly used fluorophores. Their emission maxima range from 544 nm to 637 nm, which make them ideal fluorophores for multiplexed qPCR.

2011年7月28日 · In the work described here, a panel of 23 different DNA dyes--including green, orange, and red SYTO dyes, EvaGreen, and SYBR Green--were evaluated with respect to their performance in real time PCR. Data were analyzed for reaction inhibition, effects on amplicon melting temperature, fluorescent signal strength, and reaction efficiency.

In this study, we investigate 15 different intercalating DNA dyes for their inhibitory effects on PCR, effects on DNA melting temperature and possible preferential binding to GC-rich sequences.