2021年1月14日 · Here, we developed a "PCR & Go" system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae. The core component of the "PCR & Go" system was an expression chassis, where eight promoter/terminator pairs were pre-installed into the yeast chromosome and PCR amplified gene fragments could be ...

In the present study, we aimed to develop a “PCR & Go” system based on expression chassis for direct integration and assembly of multi-gene biosynthetic pathways into the yeast genome by CRISPR/Cas9.

PCR GO通用型染料法qPCR预混液预装冻干板条是一种适用于SYBR Green染料法荧光定量PCR的原位全组分冻干试剂（八联排冻存管包装），包含快速热启动Taq DNA聚合酶、SYBR Green I荧光染料、PCR Buffer、dNTPs、Mg2+以及增强剂和稳定剂。

Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae. The core component of the “PCR & Go” system was an expression chassis, where eight promoter/terminator pairs were pre-installed into the yeast chromosome and PCR amplified gene fragments ...

pcr & go Supplementary materials to the manuscript "PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-gene Biosynthetic Pathways" Browse

Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae. The core component of the “PCR & Go” system was an expression chassis, where eight promoter/terminator pairs were pre-installed into the yeast chromos

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR. The kit is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacon probes.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The redesigned buffer system and modified reverse transcriptase offer enhanced yields and increased sensitivity …

Pcr & Go: A Pre-Installed Expression Chassis For Facile Integration Of Multi-Gene Biosynthetic Pathways Mingming Qi , Bei Zhang , Lihong Jiang , Saijuan Xu , Chang Dong , Yi-Ling Du , Zhan Zhou , Lei Huang , Zhinan Xu , Jiazhang Lian

Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae.