Shiga toxin-producing Escherichia coli (STEC)-associated hemolytic uremic syndrome (STEC-HUS) is a clinical syndrome involving hemolytic anemia (with fragmented red blood cells), low levels of platelets in the blood (thrombocytopenia), and acute kidney injury (AKI). It is the major infectious cause of AKI in children.

The Thermo Scientific™ SureTect™ Escherichia coli STEC Identification PCR Assay is part of a complete workflow for the rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145) from food samples. The assay incorporates an optimized liquid lysis step, providing a quick ...

In recent years, many diagnostic laboratories have switched to PCR for detection of stx 1 and stx 2 and other putative virulence genes (e.g., eae and ehxA) in STEC strains. Although the use of PCR improves the detection of non-O157 STEC serotypes which may be the cause of HUS, it may also lead to detection of a range of STEC strains with a ...

The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.

The BAX® System Real-Time STEC Screening assay is a rapid PCR test for the qualitative detection of Shiga toxin-producing Escherichia coli by targeting the stx and eae genes. As part of the STEC testing suite, it provides a foundational screening step …

To develop subtyping methods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex real-time polymerase chain reaction (PCR) assays with reference to 284 valid stx sequences and evaluated their ...

2024年7月3日 · Here is a selection of 4 reliable PCR methods for detecting Shiga toxin-producing E. coli (STEC) in food.

The foodproof STEC Identification LyoKit enables rapid detection and identification of eight Shiga toxin-producing E. coli serogroups (O26, O45, O103, O104, O111, O121, O145, O157) in food and environmental samples. This AOAC-validated PCR test provides reliable results for raw meat testing, offering a streamlined workflow from sample to result.

A multiplex PCR is used for the detection of stx genes from EHEC and non-EHEC strains isolated from patients of enteritis. Methods: Ten EHEC and 35 non-EHEC strains obtained from patients with diarrhoea and enteritis were studied.

A further multiplex PCR has been used to detect O157:H7 STEC; the PCR uses stx 1 - and stx 2-specific primer pairs in combination with a primer pair that recognizes a single base mutation in the β-glucoronidase-encoding uidA gene .