siRNA-mediated silencing of phosphodiesterase 4B expression …
In this study, the expression of PDE4 subtypes in primary microglial cells was assayed. Primary microglial cells were then transfected with specific small interfering RNA (siRNA) against each PDE4 subtype. PDE4 subtype A-D knockdown was confirmed by …
siRNA-mediated silencing of phosphodiesterase 4B expression …
2016年10月6日 · In this study, the expression of PDE4 subtypes in primary microglial cells was assayed. Primary microglial cells were then transfected with specific small interfering RNA (siRNA) against each PDE4 subtype. PDE4 subtype A-D knockdown was confirmed by quantitative polymerase chain reaction.
Phosphodiesterase-4D Knock-down in the Prefrontal Cortex
2015年7月10日 · Phosphodiesterase 4 (PDE4) has four isoforms (PDE4A-D) with at least 25 splice variants. PDE4 subtype nonselective inhibitors produce potent antidepressant-like and cognition-enhancing effects...
Phosphodiesterase-4D knock-out and RNA interference-mediated …
2011年1月5日 · Phosphodiesterase-4 (PDE4) plays an important role in mediating memory via the control of intracellular cAMP signaling; inhibition of PDE4 enhances memory. However, development of PDE4 inhibitors as memory enhancers has been hampered by their major side effect of emesis. PDE4 has four subtypes (PDE4 …
Inhibition of PDE4B suppresses inflammation by increasing …
2013年4月9日 · In the present study, we identified PDE4B as a key negative regulator for CYLD via selective activation of c-jun N-terminal kinase 2 (JNK2), but not JNK1, and inhibition of PDE4B significantly...
Knockdown of phosphodiesterase 4 (PDE4) mRNA using siRNA.
Using quantitative PCR, expression of mRNA for PDE4A-D was found to be successfully reduced after transfection of U937 cells with small interfering RNA (siRNA) specifi c for each of the PDE4 ...
Structures of the four subfamilies of phosphodiesterase-4 provide ...
In the present study, we rectify this by providing the crystal structures of the catalytic domains of PDE4A, PDE4B and PDE4D in complex with the PDE4 inhibitor NVP {4- [8- (3-nitrophenyl)- [1,7]naphthyridin-6-yl]benzoic acid} as well as the unliganded PDE4C structure.
RNA interference-mediated knockdown of long-form ... - PubMed
Phosphodiesterase-4 (PDE4) inhibitors enhance memory, increase hippocampal neurogenesis, and reverse amyloid-β (Aβ)-induced memory deficits. Here, we examined whether long-form PDE4D knockdown by lentiviral RNA construct containing a specific microRNA/miRNA-mir hairpin structure (4DmiRNA) reversed m …
PDE4B inhibition by nerandomilast: Effects on lung fibrosis and
2024年8月25日 · Nerandomilast (BI 1015550), a preferential PDE4B inhibitor, improved bleomycin-induced fibrotic changes in a mouse model. Nerandomilast showed acceptable tolerability and stabilised lung function in patients with IPF. Nerandomilast improves fibrotic changes in a rat bleomycin model by modulating IPF-associated signalling pathways.
2015年4月28日 · PDE4 subtypes in microglial cells and the specific contri-bution of each subtype to inflammation remain unclear. In this study, the expression of PDE4 subtypes in primary microglial cells was assayed. Primary microglial cells were then transfected with specific small interfering RNA (siRNA) against each PDE4 subtype. PDE4 subtype A-D knockdown