CHKA downregulation impairs the PI3K/AKT signaling and inhibits cell proliferation. To further establish the relationship between CHKA and the PI3K/AKT signaling pathway, we first knocked down CHKA levels in Caco2 cells using shRNA. Among the four designed shRNAs, shRNA3 showed the best knockdown effect . Therefore, it was selected for ...

PI3K-Akt信号通路是细胞中一个关键的信号传导网络，它可被多种细胞刺激或毒性损伤所激活，并在调控转录、翻译、增殖、生长和存活等基本细胞功能中扮演重要角色。

2025年1月27日 · In this study, choline kinase alpha (CHKA) and ATP-dependent 6-phosphofructokinase, muscle type (PFKM) were identified and verified as the binding targets of CQ. CQ specifically binds to CHKA, inhibits its expression and enzymatic activity, and downregulates the downstream phosphorylation of PI3K …

2016年10月11日 · Functional study demonstrated that knockdown of CHKA expression profoundly suppressed the growth and metastasis of CRC cells both in vitro and in vivo. Mechanistic investigation revealed that EGFR/PI3K/AKT pathway was …

2024年8月24日 · Our experimental results demonstrated that AGEs upregulated the choline metabolism mediated by choline kinase alpha (CHKA) through the receptor for advanced glycation end products (RAGE), activating the PI3K/AKT pathway and enhancing the malignant biological behavior of EC cells.

2017年2月1日 · CHKα1 positively regulates cell proliferation and survival via activation of PI3K/AKT and MAPK signalling pathways [11]. Its overexpression has been detected in 40–60% of human tumours [12] , and high levels of CHKα correlate with poor prognosis [13] .

CQ specifically binds to CHKA, inhibits its expression and enzymatic activity, and downregulates the downstream phosphorylation of PI3K and AKT, thereby suppressing tumor cell proliferation and inducing apoptosis.

2021年3月23日 · 磷脂酰肌醇 3-激酶（PI3K）是一种胞内磷脂酰肌醇激酶，同时具有丝氨酸/苏氨酸（Ser/Thr）激酶和磷脂酰肌醇激酶的活性，其可参与增殖、分化和葡萄糖转运等多种细胞功能的调节。

Results: CHKA increased in glioma tissues and promoted cell proliferation, invasion, and migration, while inhibiting the glioma cell apoptosis. It was also showed that CHKA promoted glioma development in vivo. GO and KEGG analysis indicated that PI3K/AKT was significantly enriched in CHKA knockdown U251 cells.

磷脂酰肌醇3-激酶 (PI3Ks)蛋白家族参与细胞存活、生长、代谢和血糖稳态等多种细胞功能的调控。 PI3K激酶活性升高通常与多种癌症相关。顾名思义，PI3Ks磷酸化磷脂酰肌醇PI（一种膜磷脂）肌醇环的第3位碳原子。PI在细胞膜组分中所占比例较小，比磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰丝氨酸含量少, 但在脑细胞膜中，含量较为丰富，达磷脂总量的10%。 PI的肌醇环上有5个可被磷酸化的位点，多种激酶可磷酸化PI肌醇环上的第4及第5位点，因而通常在这两个位点之一或两 …