2020年5月23日 · We generated pif knock out (KO) mutants using targeted gene disruption by homologous recombination. We generated KO cassettes consisting of a selection marker …

2016年6月1日 · To investigate whether Mp-PIF is involved in Mp-phy signaling, we generated knockout mutants of Mp-PIF (pif KO) using homologous recombination-based gene targeting …

2019年2月28日 · MpPIF functions as a positive regulator of FR-HIR signaling. Phenotypic analyses of Tak-2 (WT), mutant lines of MpPIF (pif ko), complemented lines (pro PIF: PIF/pif …

2021年8月23日 · This effect on gemma dormancy resembles the capacity to germinate in the dark of Mppif ko, which is a loss-of-function mutant of MpPHYTOCHROME INTERACTING …

2023年7月31日 · Phytochrome-Interacting Factors are one of the most important classes of transcription factors that regulate both developmental and external stimuli-based growth of plants.

2016年6月1日 · Mp-phy photoreversibly regulates gemma germination and downstream gene expression by interacting with Mp-PIF and targeting it for degradation in an R-dependent …

2024年9月23日 · In this study, we present the structures of full-length Arabidopsis phyB-Pfr or the constitutively active phyB Y276H mutant in complex with an N-terminal segment (residues …

Phytochromes mainly function in the nucleus and regulate sets of genes by inhibiting negatively acting basic helix-loop-helix transcription factors named PHYTOCHROME INTERACTING …

2014年8月14日 · Here, we show that in Arabidopsis, dark-induced senescence requires phytochrome-interacting transcription factors PIF4 and PIF5 (PIF4/PIF5). ELF3 and …

2022年6月16日 · We examined growth rates and cell properties of loss-of-function mutants, analyzed protein-protein interactions and performed gene expression studies using reporter …