1997年12月15日 · We expressed the gamma-subspecies of protein kinase C (gamma-PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. gamma-PKC-GFP fusion protein had enzymological p …

2000年7月10日 · In order to investigate PKCα's subcellular distribution and especially its nuclear accumulation in more detail we used fusion proteins consisting of PKCα and the green fluorescent protein (GFP). Purified GFP-PKCα from baculovirus-infected insect cells undergoes nuclear accumulation without any further stimuli in digitonin-permeabilized cells.

To elucidate subtype-specific functions of PKC, we have developed the methods to observe PKC translocation in real time and in the living state using PKC fused with fluorescent proteins, including GFP and DsRed.

Using green fluorescent protein (GFP)-tagged PKC, the dynamics of PKC translocation in response to different stimuli can be monitored in live cells and in real time. Several studies have shown that the translocation of PKC is isoform-, cell type-, and activator-specific and is tightly regulated by the various cofactors ( 12-15 ).

2023年11月7日 · 使用 C 激酶活性受体 (CKAR)（细胞内 PKC 激活的指标）来评估异丙酚诱导的细胞 PKC 激活。 我们还使用异丙酚的异构体和衍生物检查了 PKC 易位，以确定该过程中涉及的关键结构基序。 主要结果：异丙酚持续将 PKCα 常规 PKC 和 PKCδ 从新型 PKC (nPKC) 易位到质膜 (PM)。 异丙酚将 nPKC 的 PKCδ 和 PKCη 分别转移至高尔基体和内质网。 异丙酚还能诱导非典型PKC或PKC以外的蛋白质的PKCδ发生核转位，使核内外的蛋白质浓度变得均匀。 CKAR 分析 …

1997年12月15日 · We expressed the γ-subspecies of protein kinase C (γ-PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. γ-PKC–GFP fusion protein had enzymological properties very similar to that of native γ-PKC.

2007年12月14日 · To provide a dynamic method of analyzing the localization of the specific isoforms of PKC in living cells, we generated fluorescent fusion proteins of the various PKC isoforms by using the green fluorescent protein (GFP) as a fluorescent marker at the carboxyl termini of these enzymes.

We expressed delta subspecies of protein kinase C (delta-PKC) fused with green fluorescent protein (GFP) in CHO-K1 cells and observed the movement of this fusion protein in living cells after three different stimulations. The delta-PKC-GFP fusion protein had enzymological characteristics very simila …

2006年11月26日 · We created green fluorescent protein (GFP)-tagged PKC-1A and PKC-1B cDNA rescuing constructs and expressed them in motor neurons. Both PKC-1 isoforms were highly expressed throughout motor...

PKC-dependent and PKC-independent PKD activation loop phosphorylation in response to vasopressin stimulation and to different concentrations of bombesin. COS-7 cells were transiently co-transfected with the GFP-PKD and expression constructs encoding either the bombesin/GRP receptor or the arginine vasopressin receptor type 1A.